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Mouse Anti-UBB Recombinant Antibody (1F5) (CBMAB-A9698-LY)

The product is antibody recognizes UBB. The antibody 1F5 immunoassay techniques such as: WB, ELISA.
See all UBB antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1F5
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
UBB (AAH09301, 1 a.a. ~ 76 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ubiquitin B
Introduction
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq]
Entrez Gene ID
7314
UniProt ID
P0CG47
Alternative Names
FLJ25987; MGC8385
Function
Ubiquitin
Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Biological Process
Biological Process energy homeostasis Source:Ensembl
Biological Process fat pad development Source:Ensembl
Biological Process female gonad development Source:Ensembl
Biological Process female meiosis I Source:Ensembl
Biological Process hypothalamus gonadotrophin-releasing hormone neuron development Source:Ensembl
Biological Process male meiosis I Source:Ensembl
Biological Process mitochondrion transport along microtubule Source:MGI1 Publication
Biological Process modification-dependent protein catabolic process Source:GO_Central1 Publication
Biological Process neuron projection morphogenesis Source:MGI1 Publication
Biological Process positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source:MGI1 Publication
Biological Process positive regulation of protein monoubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process positive regulation of protein ubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process protein ubiquitination Source:GO_Central1 Publication
Biological Process regulation of mitochondrial membrane potential Source:MGI1 Publication
Biological Process regulation of neuron death Source:MGI1 Publication
Biological Process regulation of proteasomal protein catabolic process Source:MGI1 Publication
Biological Process seminiferous tubule development Source:Ensembl
Cellular Location
Ubiquitin
Cytoplasm
Nucleus
Mitochondrion outer membrane
PTM
Ubiquitin
Phosphorylated at Ser-65 by PINK1 during mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291, PubMed:26161729).
Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291, PubMed:26161729).
Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).
Ubiquitin
Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.
Ubiquitin
(Microbial infection) Mono-ADP-ribosylated at Thr-66 by the C.violaceum CteC virulence factor. ADP-ribosylation causes the shutdown of polyubiquitin synthesis and disrupts the recognition and reversal of polyubiquitin.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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