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Mouse Anti-UBE2C Recombinant Antibody (3B1) (CBMAB-A9702-LY)

The product is antibody recognizes UBE2C. The antibody 3B1 immunoassay techniques such as: WB, ELISA.
See all UBE2C antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B1
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
UBE2C (AAH50736, 1 a.a. ~ 179 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ubiquitin Conjugating Enzyme E2 C
Introduction
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
UniProt ID
Alternative Names
UBCH10; dJ447F3.2
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Biological Process
Biological Process anaphase-promoting complex-dependent catabolic process Source:UniProtKB2 Publications
Biological Process cell division Source:UniProtKB-KW
Biological Process exit from mitosis Source:UniProtKB1 Publication
Biological Process free ubiquitin chain polymerization Source:UniProtKB1 Publication
Biological Process positive regulation of exit from mitosis Source:UniProtKB1 Publication
Biological Process positive regulation of ubiquitin protein ligase activity Source:UniProtKB1 Publication
Biological Process protein K11-linked ubiquitination Source:UniProtKB3 Publications
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process protein ubiquitination Source:UniProtKB1 Publication
Biological Process regulation of mitotic metaphase/anaphase transition Source:GO_Central1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB2 Publications
Cellular Location
anaphase-promoting complex
cytosol
nucleoplasm
nucleus
plasma membrane
ubiquitin ligase complex
PTM
Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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