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Mouse Anti-UBE2N Recombinant Antibody (3G1-D10) (CBMAB-U0138-FY)

This product is mouse antibody that recognizes UBE2N. The antibody 3G1-D10 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all UBE2N antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3G1-D10
Antibody Isotype
IgG2b, Κ
Application
ELISA, IF, WB

Basic Information

Immunogen
Full length recombinant protein with GST tag. MW of the GST tag alone is 26 kD. Immunogen sequence: MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI.
Specificity
Human
Antibody Isotype
IgG2b, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-152

Target

Full Name
Ubiquitin Conjugating Enzyme E2 N
Introduction
This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Entrez Gene ID
7334
UniProt ID
P61088
Alternative Names
UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
Function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175, PubMed:31006531).
UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.
Biological Process
Biological Process DNA double-strand break processing Source:HGNC-UCL1 Publication
Biological Process double-strand break repair via homologous recombination Source:HGNC-UCL1 Publication
Biological Process histone ubiquitination Source:HGNC-UCL1 Publication
Biological Process positive regulation of DNA repair Source:HGNC-UCL1 Publication
Biological Process positive regulation of double-strand break repair Source:ComplexPortal1 Publication
Biological Process positive regulation of histone modification Source:HGNC-UCL1 Publication
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signaling Source:HGNC-UCL1 Publication
Biological Process positive regulation of intracellular signal transduction Source:ComplexPortal1 Publication
Biological Process positive regulation of NF-kappaB transcription factor activity Source:UniProtKB1 Publication
Biological Process positive regulation of protein K63-linked ubiquitination Source:ComplexPortal2 Publications
Biological Process positive regulation of ubiquitin-protein transferase activity Source:HGNC-UCL1 Publication
Biological Process postreplication repair Source:HGNC-UCL1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB4 Publications
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process protein ubiquitination Source:HGNC-UCL1 Publication
Biological Process regulation of DNA repair Source:ProtInc1 Publication
Biological Process regulation of histone ubiquitination Source:HGNC-UCL1 Publication
Biological Process T cell receptor signaling pathway Source:UniProtKB1 Publication
Cellular Location
Nucleus
Cytoplasm
PTM
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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