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Mouse Anti-UBE2O Recombinant Antibody (CBFYU-143) (CBMAB-U0143-FY)

This product is mouse antibody that recognizes UBE2O. The antibody CBFYU-143 can be used for immunoassay techniques such as: WB, IP, ELISA.
See all UBE2O antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYU-143
Antibody Isotype
IgG2a, к
Application
WB, IP, ELISA

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 kD. Immunogen sequence: VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC SPDTQCSRDH SMEDPDKKGE SKTKSEAESA SPEETPDGSA SPVEMQDEGA EEPHEAGEQL PPFLLKEGRD.
Specificity
Human
Antibody Isotype
IgG2a, к
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 361-470

Target

Full Name
ubiquitin-conjugating enzyme E2O
Introduction
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity.
Entrez Gene ID
63893
UniProt ID
Q9C0C9
Alternative Names
E2-230K
Function
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950).
Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138).
Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153).
Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950).
Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
Biological Process
Biological Process negative regulation of apoptotic process Source:GO_Central1 Publication
Biological Process positive regulation of BMP signaling pathway Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB1 Publication
Biological Process retrograde transport, endosome to Golgi Source:UniProtKB1 Publication
Cellular Location
Cytoplasm
Nucleus
Mainly localizes to the cytoplasm.
PTM
Phosphorylated. Phosphorylation affects subcellular location.
Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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