Mouse Anti-UBE2O Recombinant Antibody (CBFYU-143) (CBMAB-U0143-FY)

Name: Mouse Anti-UBE2O Recombinant Antibody (CBFYU-143)
SKU: CBMAB-U0143-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

CBFYU-143

Application

WB, IP, ELISA

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 kD. Immunogen sequence: VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC SPDTQCSRDH SMEDPDKKGE SKTKSEAESA SPEETPDGSA SPVEMQDEGA EEPHEAGEQL PPFLLKEGRD.

Specificity

Human

Antibody Isotype

IgG2a, к

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 361-470

More Infomation

Target

Full Name

ubiquitin-conjugating enzyme E2O

Introduction

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity.

Entrez Gene ID

63893

UniProt ID

Q9C0C9

Alternative Names

E2-230K

Function

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950).
Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138).
Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153).
Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950).
Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).

Biological Process

Biological Process negative regulation of apoptotic process Source:GO_Central1 Publication
Biological Process positive regulation of BMP signaling pathway Source:UniProtKB1 Publication
Biological Process protein K63-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB1 Publication
Biological Process retrograde transport, endosome to Golgi Source:UniProtKB1 Publication

Cellular Location

Cytoplasm
Nucleus
Mainly localizes to the cytoplasm.

PTM

Phosphorylated. Phosphorylation affects subcellular location.
Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-UBE2O Recombinant Antibody (2C10) (CAT#: CBMAB-A9724-LY)

Isotype control

For research use only. Not intended for any clinical use.

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