Mouse Anti-UBE3A Monoclonal Antibody (V2-888) (CBMAB-0283-LY)

Name: Mouse Anti-UBE3A Monoclonal Antibody (V2-888)
SKU: CBMAB-0283-LY
Rating: 5 (1 reviews)

See all UBE3A antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-888

Application

IP, MA, WB

Immunogen

Recombinant peptide (Full Length)

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

UBE3A

Introduction

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses.

Entrez Gene ID

7337

UniProt ID

Q05086

Alternative Names

ANCR; AS; E6-AP; EPVE6AP; FLJ26981; HPVE6A

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates (PubMed:10373495, PubMed:16772533, PubMed:19204938, PubMed:19233847, PubMed:19325566, PubMed:19591933, PubMed:22645313, PubMed:24273172, PubMed:24728990).
Several substrates have been identified including the BMAL1, ARC, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed:10373495, PubMed:19204938, PubMed:19325566, PubMed:19591933, PubMed:22645313, PubMed:24728990).
Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins (PubMed:19233847).
Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation (PubMed:24728990).
Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation (PubMed:16772533).
Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (By similarity).
Synergizes with WBP2 in enhancing PGR activity (PubMed:16772533).
(Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses.

Biological Process

Biological Process androgen receptor signaling pathway Source:Ensembl
Biological Process brain development Source:ProtInc1 Publication
Biological Process modulation of chemical synaptic transmission Source:Ensembl
Biological Process ovarian follicle development Source:Ensembl
Biological Process positive regulation of Golgi lumen acidification Source:Ensembl
Biological Process positive regulation of phosphatidylinositol 3-kinase signaling Source:Ensembl
Biological Process positive regulation of protein ubiquitination Source:CACAO1 Publication
Biological Process positive regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process progesterone receptor signaling pathway Source:UniProtKB1 Publication
Biological Process prostate gland growth Source:Ensembl
Biological Process protein autoubiquitination Source:FlyBase1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process proteolysis Source:ProtInc1 Publication
Biological Process regulation of circadian rhythm Source:UniProtKB1 Publication
Biological Process regulation of ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Biological Process response to progesterone Source:UniProtKB1 Publication
Biological Process rhythmic process Source:UniProtKB-KW
Biological Process sperm entry Source:Ensembl
Biological Process ubiquitin-dependent protein catabolic process Source:ProtInc1 Publication

Cellular Location

Cytoplasm
Nucleus

Involvement in disease

Angelman syndrome (AS):
A neurodevelopmental disorder characterized by severe motor and intellectual retardation, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, open-mouthed expression revealing the tongue.

PTM

A neurodevelopmental disorder characterized by severe motor and intellectual retardation, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, open-mouthed expression revealing the tongue.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-UBE3A Monoclonal Antibody (V2-888)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-UBE3A Monoclonal Antibody (1A10) (CAT#: CBMAB-0282-LY)

Mouse Anti-UBE3A Recombinant Antibody (CBFYU-166) (CAT#: CBMAB-U0166-FY)

Mouse Anti-UBE3A Recombinant Antibody (CBFYE-1683) (CAT#: CBMAB-E2356-FY)

Mouse Anti-UBE3A (AA 51-151) Recombinant Antibody (CBFYE-0010) (CAT#: CBMAB-E0018-FY)

Mouse Anti-UBE3A Recombinant Antibody (CBFYU-171) (CAT#: CBMAB-U0171-FY)

Rabbit Anti-UBE3A Recombinant Antibody (CBFYU-169) (CAT#: CBMAB-U0169-FY)

Mouse Anti-UBE3A Recombinant Antibody (CBFYU-170) (CAT#: CBMAB-U0170-FY)

Mouse Anti-UBE3A Recombinant Antibody (EX-8) (CAT#: CBMAB-0912CQ)

Mouse Anti-UBE3A Recombinant Antibody (CBFYU-168) (CAT#: CBMAB-U0168-FY)

Mouse Anti-UBE3A Recombinant Antibody (CBFYE-1682) (CAT#: CBMAB-E2355-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry