Rabbit Anti-UIMC1 Recombinant Antibody (D1T6Q) (CBMAB-CP2210-LY)

Name: Rabbit Anti-UIMC1 Recombinant Antibody (D1T6Q)
SKU: CBMAB-CP2210-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Rabbit

Clone

D1T6Q

Application

WB, IF (ICC)

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly321 of human RAP80 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ubiquitin Interaction Motif Containing 1

Introduction

This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Entrez Gene ID

51720

UniProt ID

Q96RL1

Alternative Names

Ubiquitin Interaction Motif Containing 1; Retinoid X Receptor-Interacting Protein 110; Receptor-Associated Protein 80; RAP80; Ubiquitin Interaction Motif-Containing Protein 1; Receptor Associated Protein 80; BRCA1-A Complex Subunit RAP80; X2HRIP110; RXRIP110;

Function

Ubiquitin-binding protein (PubMed:24627472).
Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.38). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

Biological Process

Biological Process chromatin organization Source:UniProtKB-KW
Biological Process double-strand break repair Source:UniProtKB5 Publications
Biological Process histone H2A K63-linked deubiquitination Source:UniProtKB1 Publication
Biological Process mitotic G2 DNA damage checkpoint signaling Source:UniProtKB5 Publications
Biological Process mitotic G2/M transition checkpoint Source:ComplexPortal1 Publication
Biological Process negative regulation of DNA-templated transcription Source:HGNC-UCL1 Publication
Biological Process positive regulation of DNA repair Source:UniProtKB5 Publications
Biological Process regulation of DNA repair Source:ComplexPortal1 Publication
Biological Process response to ionizing radiation Source:UniProtKB5 Publications

Cellular Location

Nucleus
Localizes at sites of DNA damage at double-strand breaks (DSBs).

PTM

Sumoylated.
Phosphorylated upon DNA damage by ATM or ATR.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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