Sign in or Register   Sign in or Register
  |  

Mouse Anti-UPF1 Recombinant Antibody (4G3) (CBMAB-R4087-CN)

This product is a Mouse antibody that recognizes UPF1. The antibody 4G3 can be used for immunoassay techniques such as: ELISA, WB.
See all UPF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4G3
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa. 1019-1117, from human UPF1 (NP_002902) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2

Target

Full Name
UPF1, RNA Helicase And ATPase
Introduction
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Entrez Gene ID
5976
UniProt ID
Q92900
Alternative Names
UPF1, RNA Helicase And ATPase; Regulator Of Nonsense Transcripts 1; Up-Frameshift Suppressor 1 Homolog; Nonsense MRNA Reducing Factor 1; ATP-Dependent Helicase RENT1; HUPF1; RENT1; NORF1; Smg-2 Homolog, Nonsense Mediated MRNA Decay Factor (C. Elegans); UPF1 Regulator Of Nonsense Transcripts Homolog (Yeast); Smg-2 Homolog, Nonsense Mediated MRNA Decay Factor;
Function
RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs (PubMed:11163187, PubMed:16086026, PubMed:18172165, PubMed:21145460, PubMed:21419344, PubMed:24726324).
Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD (PubMed:11544179, PubMed:25220460).
Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (PubMed:19417104).
In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD (PubMed:21419344).
Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors (PubMed:12554878).
UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways (PubMed:18447585).
Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2 (PubMed:16086026, PubMed:18172165).
For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585, PubMed:25220460).
The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (PubMed:21145460).
Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA harboring long 3'UTR by inducing the NMD machinery (By similarity).
Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA (PubMed:30218034).
Biological Process
Biological Process 3'-UTR-mediated mRNA destabilization Source:UniProtKB1 Publication
Biological Process cell cycle phase transition Source:UniProtKB1 Publication
Biological Process cellular response to interleukin-1 Source:UniProtKB1 Publication
Biological Process cellular response to lipopolysaccharide Source:UniProtKB1 Publication
Biological Process DNA duplex unwinding Source:UniProtKB1 Publication
Biological Process DNA repair Source:HGNC-UCL1 Publication
Biological Process DNA replication Source:HGNC-UCL1 Publication
Biological Process histone mRNA catabolic process Source:UniProtKB1 Publication
Biological Process mRNA export from nucleus Source:HGNC-UCL1 Publication
Biological Process nuclear-transcribed mRNA catabolic process Source:UniProtKB1 Publication
Biological Process nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay Source:UniProtKB1 Publication
Biological Process nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source:UniProtKB1 Publication
Biological Process positive regulation of mRNA catabolic process Source:UniProtKB1 Publication
Biological Process regulation of telomere maintenance Source:BHF-UCL1 Publication
Biological Process regulation of translational termination Source:UniProtKB1 Publication
Biological Process telomere maintenance via semi-conservative replication Source:BHF-UCL1 Publication
Cellular Location
Cytoplasm
Cytoplasm, P-body
Nucleus
Cytoplasm, perinuclear region
Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. Localized in the chromatoid bodies of round spermatids (By similarity).
PTM
Phosphorylated by SMG1; required for formation of mRNA surveillance complexes.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-UPF1 Recombinant Antibody (4G3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare