Rabbit Anti-USP24 Recombinant Antibody (
11E3) (V2LY-0725-LY1008)

Name: Rabbit Anti-USP24 Recombinant Antibody (11E3)
SKU: V2LY-0725-LY1008
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-USP24 Recombinant Antibody (11E3) (V2LY-0725-LY1008) in IF

Immunofluorescence staining of HepG2 cell with V2LY-0725-LY1008 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-USP24 Recombinant Antibody (11E3) (V2LY-0725-LY1008) in FC

Overlay Peak curve showing HepG2 cells stained with V2LY-0725-LY1008 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

11E3

Application

ELISA, IF, FC

Immunogen

A synthesized peptide derived from human USP24.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ubiquitin specific peptidase 24

Entrez Gene ID

23358

UniProt ID

Q9UPU5

Function

Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron.

Biological Process

Biological Process protein deubiquitination Source:GO_Central1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

cytosol
nucleoplasm
nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-USP24 Recombinant Antibody (CBXU-033) (CAT#: CBMAB-U0149-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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