Mouse Anti-VAMP8 Recombinant Antibody (1414CT354.12.23.93) (CBMAB-0942CQ)

Name: Mouse Anti-VAMP8 Recombinant Antibody (1414CT354.12.23.93)
SKU: CBMAB-0942CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1414CT354.12.23.93

Application

Immunogen

Human VAMP8 aa 2-24 (N terminal)

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Vesicle Associated Membrane Protein 8

Introduction

This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Entrez Gene ID

8673

UniProt ID

Q9BV40

Alternative Names

EDB; VAMP-8

Function

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604).
Also required for dense-granule secretion in platelets (PubMed:12130530).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity).
Involved in the homotypic fusion of early and late endosomes (By similarity).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (PubMed:31694946).

Biological Process

Biological Process autophagosome maturation Source:UniProtKB1 Publication
Biological Process autophagosome membrane docking Source:GO_Central1 Publication
Biological Process defense response to virus Source:UniProtKB-KW
Biological Process mucus secretion Source:UniProtKB1 Publication
Biological Process negative regulation of secretion by cell Source:UniProtKB1 Publication
Biological Process positive regulation of histamine secretion by mast cell Source:UniProtKB1 Publication
Biological Process protein transport Source:UniProtKB-KW
Biological Process regulation of protein localization to plasma membrane Source:UniProtKB1 Publication
Biological Process SNARE complex assembly Source:GO_Central1 Publication
Biological Process vesicle fusion Source:GO_Central1 Publication
Biological Process viral entry into host cell Source:MGI1 Publication

Cellular Location

Lysosome membrane
Early endosome membrane
Late endosome membrane
Cell membrane
Zymogen granule membrane
Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity).
Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (By similarity).

Topology

Cytoplasmic: 1-75
Helical: 76-96
Vesicular: 97-100

PTM

(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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