Mouse Anti-VIM Recombinant Antibody (CF475) (CBMAB-FT059LY)

Name: Mouse Anti-VIM Recombinant Antibody (CF475)
SKU: CBMAB-FT059LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CF475

Application

ELISA, WB, IHC

Immunogen

vimentin

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

50% glycerol

Preservative

0.02% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Vimentin

Introduction

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Entrez Gene ID

Human	7431
Mouse	22352
Rat	81818

UniProt ID

Human	P08670
Mouse	P20152
Rat	P31000

Alternative Names

FLJ36605, VIM, Vimentin

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Biological Process

Astrocyte developmentIEA:Ensembl
Bergmann glial cell differentiationIEA:Ensembl
Cellular response to interferon-gammaIEA:Ensembl
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to muramyl dipeptideManual Assertion Based On ExperimentIMP:UniProtKB
Intermediate filament organizationISS:UniProtKB
Lens fiber cell developmentIEA:Ensembl
Negative regulation of neuron projection developmentIEA:Ensembl
Positive regulation of collagen biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of translationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of mRNA stabilityManual Assertion Based On ExperimentIMP:UniProtKB
SMAD protein signal transductionIEA:Ensembl

Cellular Location

Cytoplasm
Cytoplasm, cytoskeleton
Nucleus matrix
Cell membrane

Involvement in disease

Cataract 30, multiple types (CTRCT30):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.

PTM

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
Phosphorylated by STK33 (PubMed:18811945).
Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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