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Mouse Anti-WDR61 Recombinant Antibody (3E5-1A12) (CBMAB-W0097-WJ)

This product is a mouse antibody that recognizes WDR61. The antibody 3E5-1A12 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all WDR61 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3E5-1A12
Antibody Isotype
IgG2b, Κ
Application
ELISA, IF, WB

Basic Information

Immunogen
REC14 (AAH10080, aa1-305) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
WD Repeat Domain 61
Introduction
WDR61 is a protein-coding gene. WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis. Its related pathways are Metabolism of proteins and Gene Expression. WDR38 is an important paralog of this gene.
Entrez Gene ID
80349
UniProt ID
Q9GZS3
Alternative Names
SKI8; REC14
Function
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (PubMed:16307923, PubMed:19952111, PubMed:20178742).
Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (PubMed:16024656).
Biological Process
Biological Process histone H3-K4 methylation Source:GO_Central1 Publication
Biological Process histone H3-K4 trimethylation Source:UniProtKB1 Publication
Biological Process negative regulation of myeloid cell differentiation Source:UniProtKB1 Publication
Biological Process positive regulation of histone H3-K4 methylation Source:UniProtKB1 Publication
Biological Process positive regulation of histone H3-K79 methylation Source:UniProtKB1 Publication
Biological Process transcription elongation by RNA polymerase II promoter Source:GO_Central1 Publication
Biological Process Wnt signaling pathway Source:UniProtKB-KW
Cellular Location
Nucleus
Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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