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Mouse Anti-WIPI1 Recombinant Antibody (3C1) (CBMAB-W0088-WJ)

This product is a mouse antibody that recognizes WIPI1. The antibody 3C1 can be used for immunoassay techniques such as: ELISA, WB.
See all WIPI1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3C1
Antibody Isotype
IgG2b, Κ
Application
ELISA, WB

Basic Information

Immunogen
WIPI1 (NP_060453, aa348-446-) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K.
Specificity
Human
Antibody Isotype
IgG2b, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
WD repeat domain, phosphoinositide interacting 1
Introduction
WIPI1 is a protein-coding gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. Among its related pathways are Unfolded Protein Response (UPR) and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include receptor binding and estrogen receptor binding. WIPI2 is an important paralog of this gene.
Entrez Gene ID
55062
UniProt ID
Q5MNZ9
Alternative Names
ATG18; ATG18A; WIPI49
Function
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:15602573, PubMed:20114074, PubMed:20484055, PubMed:20639694, PubMed:23088497, PubMed:28561066, PubMed:31271352).
Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (PubMed:28561066).
Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated (PubMed:28561066).
Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes (PubMed:28561066, PubMed:31271352, PubMed:33499712).
There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed:28561066).
Together with WDR45/WIPI4, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352).
Involved in xenophagy of Staphylococcus aureus (PubMed:22829830).
Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation (PubMed:22829830).
Also plays a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy (PubMed:21317285).
May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (PubMed:15020712).
Biological Process
Biological Process autophagosome assembly Source:UniProtKB1 Publication
Biological Process autophagy Source:UniProtKB2 Publications
Biological Process autophagy of mitochondrion Source:GO_Central1 Publication
Biological Process autophagy of nucleus Source:GO_Central1 Publication
Biological Process cellular response to starvation Source:UniProtKB1 Publication
Biological Process positive regulation of autophagosome assembly Source:UniProtKB1 Publication
Biological Process protein lipidation Source:GO_Central1 Publication
Biological Process protein localization to phagophore assembly site Source:GO_Central1 Publication
Biological Process vesicle targeting, trans-Golgi to endosome Source:UniProtKB1 Publication
Cellular Location
Golgi apparatus, trans-Golgi network
Endosome
Cytoplasmic vesicle, clathrin-coated vesicle
Preautophagosomal structure membrane
Cytoplasm, cytoskeleton
Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. Upon starvation-induced autophagy, accumulates at subcellular structures in the cytoplasm: enlarged vesicular and lasso-like structures, and large cup-shaped structures predominantly around the nucleus. Recruitment to autophagic membranes is controlled by MTMR14. Labile microtubules specifically recruit markers of autophagosome formation like WIPI1, whereas mature autophagosomes may bind to stable microtubules.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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