Rabbit Anti-XAF1 Recombinant Antibody (E1E4O) (CBMAB-CP0001-LY)

Name: Rabbit Anti-XAF1 Recombinant Antibody (E1E4O)
SKU: CBMAB-CP0001-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

E1E4O

Application

WB, IP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val210 of human XAF1 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

XIAP Associated Factor 1

Introduction

This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

54739

UniProt ID

Q6GPH4

Function

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.

Biological Process

Biological Process apoptotic process Source:UniProtKB-KW
Biological Process response to interferon-beta Source:BHF-UCL1 Publication

Cellular Location

Cytoplasm
Nucleus
Mitochondrion
Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
Isoform 1
Nucleus
Isoform 5
Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

XAF1 Matched Antibody Pair (1308) (CAT#: APMAB-1308LY)

Related Products

Mouse Anti-XAF1 Recombinant Antibody (CBYCX-112) (CAT#: CBMAB-X0077-YC)

Mouse Anti-XAF1 Recombinant Antibody (3E5) (CAT#: CBMAB-A9959-LY)

XAF1 Matched Antibody Pair (1308) (CAT#: APMAB-1308LY)

Mouse Anti-XAF1 Recombinant Antibody (CBYCX-111) (CAT#: CBMAB-X0076-YC)

Mouse Anti-XAF1 Recombinant Antibody (CBYCX-110) (CAT#: CBMAB-X0075-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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