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Mouse Apoptosis Signaling Pathway Antibody Array (Membrane, 17 Targets) (Y0223) (AbAr-0223-YC)

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Specifications

Target Array
Apoptosis Signaling Pathway Array
Target
AKT (pSer473), CHK1 (pSer296), JNK (pThr183), SMAD2 (pSer245), ATM (pSer1981), eIF-2a (pSer52), NF-kB P65 (pSer536), TAK1 (pSer412), BAD (pSer112), ERK1/2 (pThr202), p27 (pThr198), Caspase-3 (pAsp175), HSP27 (pSer82), p38 (pThr180, pTyr182), CASP7 (pAsp198), IKBa (pSer32), P53 (pSer15)
Species Reactivity
Mouse
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Conduct membrane blocking procedures.
Incubate with the provided sample.
Incubate using the detection antibody cocktail.
Proceed with incubation using HRP-anti-IgG antibodies.
Engage in incubation with detection buffer solutions.
Capture images utilizing a chemiluminescent imaging system.
Perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. The average adult human loses between 50 and 70 billion cells each day due to apoptosis. For an average human child between the ages of 8 to 14 year old approximately 20 to 30 billion cells die per day.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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