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Human Autophagy Antibody Array (Membrane, 20 Targets) (Y0394) (AbAr-0394-YC)


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Specifications

Target Array
Autophagy Array
Target
ATG12, ATG7, ATG10, ATG13, ATG3, ATG4A, ATG4B, ATG5, Beclin, BNIP3L, DDR2, GABARAP, LAMP1, LC3a, LC3B, NBS1, P62, RHEB, MSK1, Alpha-synuclein
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Conduct blocking of the array membrane surface.
Proceed with incubation of the sample.
Incubate with a biotinylated detection antibody cocktail.
Continue with incubation using HRP-streptavidin.
Engage in incubation with detection buffers.
Capture images using a chemiluminescent imaging system.
Perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Autophagy is a self-degradative process that is important for balancing sources of energy at critical times in development and in response to nutrient stress. Autophagy also plays a housekeeping role in removing misfolded or aggregated proteins, clearing damaged organelles, such as mitochondria, endoplasmic reticulum and peroxisomes, as well as eliminating intracellular pathogens. Thus, autophagy is generally thought of as a survival mechanism, although its deregulation has been linked to non-apoptotic cell death. Autophagy can be either non-selective or selective in the removal of specific organelles, ribosomes and protein aggregates, although the mechanisms regulating aspects of selective autophagy are not fully worked out.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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