CALR Matched Antibody Pair (173) (APMAB-173LY)

Name: CALR Matched Antibody Pair (173)
SKU: APMAB-173LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet References Q & As Review & reward Protocols Associated Products

Specifications

ApplIcation

Sandwich ELISA

Specificity

Human

Capture Antibody

Mouse anti-CALR monoclonal, IgG1 antibody, 100 ug

Detection Antibody

Anti-CALR Rabbit polyclonal antibody, 50 ug

Dilutions

10 ng/ml-100 ng/ml

Format

Liquid

Storage

Aliquot and store at -20°Cor -80°C. Avoid freeze-thaw cycles.

Introduction

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Alternative Names

Calreticulin; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; Calregulin; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n;

Entrez Gene ID

811

UniProt ID

P27797

More Infomation

Fucikova, J., Spisek, R., Kroemer, G., & Galluzzi, L. (2021). Calreticulin and cancer. Cell research, 31(1), 5-16.

Biwer, L. A., Askew-Page, H. R., Hong, K., Milstein, J., Johnstone, S. R., Macal, E., ... & Isakson, B. E. (2020). Endothelial calreticulin deletion impairs endothelial function in aged mice. American Journal of Physiology-Heart and Circulatory Physiology, 318(5), H1041-H1048.

Masubuchi, N., Araki, M., Yang, Y., Hayashi, E., Imai, M., Edahiro, Y., ... & Komatsu, N. (2020). Mutant calreticulin interacts with MPL in the secretion pathway for activation on the cell surface. Leukemia, 34(2), 499-509.

Liu, P., Zhao, L., Loos, F., Marty, C., Xie, W., Martins, I., ... & Kroemer, G. (2020). Immunosuppression by mutated calreticulin released from malignant cells. Molecular cell, 77(4), 748-760.

Araki, M., Yang, Y., Imai, M., Mizukami, Y., Kihara, Y., Sunami, Y., ... & Komatsu, N. (2019). Homomultimerization of mutant calreticulin is a prerequisite for MPL binding and activation. Leukemia, 33(1), 122-131.

Salati, S., Genovese, E., Carretta, C., Zini, R., Bartalucci, N., Prudente, Z., ... & Manfredini, R. (2019). Calreticulin Ins5 and Del52 mutations impair unfolded protein and oxidative stress responses in K562 cells expressing CALR mutants. Scientific reports, 9(1), 1-14.

Bozkus, C. C., Roudko, V., Finnigan, J. P., Mascarenhas, J., Hoffman, R., Iancu-Rubin, C., & Bhardwaj, N. (2019). Immune checkpoint blockade enhances shared neoantigen-induced T-cell immunity directed against mutated calreticulin in myeloproliferative neoplasms. Cancer discovery, 9(9), 1192-1207.

Elf, S., Abdelfattah, N. S., Baral, A. J., Beeson, D., Rivera, J. F., Ko, A., ... & Mullally, A. (2018). Defining the requirements for the pathogenic interaction between mutant calreticulin and MPL in MPN. Blood, The Journal of the American Society of Hematology, 131(7), 782-786.

Biwer, L. A., Good, M. E., Hong, K., Patel, R. K., Agrawal, N., Looft-Wilson, R., ... & Isakson, B. E. (2018). Non–Endoplasmic Reticulum–Based Calr (Calreticulin) Can Coordinate Heterocellular Calcium Signaling and Vascular Function. Arteriosclerosis, thrombosis, and vascular biology, 38(1), 120-130.

Holmström, M. O., Martinenaite, E., Ahmad, S. M., Met, Ö., Friese, C., Kjaer, L., ... & Andersen, M. H. (2018). The calreticulin (CALR) exon 9 mutations are promising targets for cancer immune therapy. Leukemia, 32(2), 429-437.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CALR protein, hFc Tag (CAT#: V2LY-0526-LY2517)

Human Recombinant CALR protein, His Tag (CAT#: V2LY-0526-LY2518)

Human Recombinant CALR protein, His Tag-1 (CAT#: V2LY-0526-LY2519)

Human Recombinant CALR protein (CAT#: V2LY-0526-LY2520)

Human Recombinant CALR protein-1 (CAT#: V2LY-0526-LY2521)

Human Recombinant CALR protein-2 (CAT#: V2LY-0526-LY2522)

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Mouse Anti-CALR Recombinant Antibody (1G6A7) (CAT#: CBMAB-1291-CN)

Mouse Anti-CALR Recombinant Antibody (CBYY-C0276) (CAT#: CBMAB-C0886-YY)

Mouse Anti-CALR Recombinant Antibody (CBFYC-0762) (CAT#: CBMAB-C0817-FY)

Mouse Anti-CALR Recombinant Antibody (1G11-1A9) (CAT#: CBMAB-C0534-LY)

Mouse Anti-CALR Recombinant Antibody (CBXC-1111) (CAT#: CBMAB-C1377-CQ)

Mouse Anti-CALR Recombinant Antibody (CBT2344) (CAT#: V2LY-0625-LY2160)

Mouse Anti-CALR Recombinant Antibody (CBFYC-0761) (CAT#: CBMAB-C0816-FY)

Human Recombinant CALR protein-1 (CAT#: V2LY-0526-LY2521)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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