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Human Cancer Discovery Antibody Array (Membrane, 20 Targets) (Y0192) (AbAr-0192-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Cancer Discovery Antibody Array detects 20 cytokines related to cancer biology. It is suitable for all liquid sample types.
Specifications
Target Array
Cancer Discovery Array
Target
Alpha-fetoprotein, CRP (C-Reactive Protein), HGF, PAI-1, Beta-2 Microglobulin, DKK-1, ICAM-1 (CD54), PSA-free, CA125, Ferritin, IL-8 (CXCL8), RANTES (CCL5), CA19-9, Galectin-3, MMP-2, TIMP-1, CEA, GDF-15, Osteopontin (SPP1), VEGF-A
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Cytokines that are released in response to infection, inflammation and immunity can function to inhibit cancer development and progression. Alternatively, cancer cells can respond to host-derived cytokines that promote growth, attenuate apoptosis and facilitate invasion and metastasis.
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For Research Use Only. Not For Clinical Use.