Canine Recombinant ANGPTL1 protein, hFc Tag (V2LY-0526-LY1204)

Name: Canine Recombinant ANGPTL1 protein, hFc Tag
SKU: V2LY-0526-LY1204
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Canine

Tag

hFc Tag

Protein Construction

This product is Canine Recombinant ANGPTL1 protein, hFc Tag consist of Amino Acid: 197-491 and predicts a molecular mass of 62.4 kDa.

Molecule Mass

62.4 kDa

Sequence

Amino Acid: 197-491

Species

Canine

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Angiopoietin Like 1

Biological Process

Angiogenesis Source: GO_Central
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Secreted

Fan, H., Huang, L., Zhuang, X., Ai, F., & Sun, W. (2019). Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress slug expression. Cytotechnology, 71(1), 35-44.

Yan, Q., Jiang, L., Liu, M., Yu, D., Zhang, Y., Li, Y., ... & Guan, X. Y. (2017). ANGPTL1 interacts with integrin α1β1 to suppress HCC angiogenesis and metastasis by inhibiting JAK2/STAT3 signaling. Cancer research, 77(21), 5831-5845.

Kuo, T. C., Tan, C. T., Chang, Y. W., Hong, C. C., Lee, W. J., Chen, M. W., ... & Kuo, M. L. (2017). Angiopoietin-like protein 1 suppresses SLUG to inhibit cancer cell motility. The Journal of clinical investigation, 127(1), 402-402.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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