Canine Recombinant CD40 protein-1 (V2LY-0526-LY1222)

Name: Canine Recombinant CD40 protein-1
SKU: V2LY-0526-LY1222
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Canine

Protein Construction

This product is Canine Recombinant CD40 protein consist of Amino Acid: 1-194 and predicts a molecular mass of 19.9 kDa.

Molecule Mass

19.9 kDa

Sequence

Amino Acid: 1-194

Species

Canine

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD40 Molecule

Alternative Names

CD40 Molecule; CD40 Molecule, TNF Receptor Superfamily Member 5; CD40L Receptor; TNFRSF5; CDW40; Bp50; Tumor Necrosis Factor Receptor Superfamily, Member 5;

Function

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
Involved in immunoglobulin class switching (By similarity).
CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Biological Process

Activation of JUN kinase activity Source: UniProtKB
B cell differentiation Source: Ensembl
B cell proliferation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Inflammatory response Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Isotype switching Source: UniProtKB
Leukocyte cell-cell adhesion Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Platelet activation Source: UniProtKB
Positive regulation of endothelial cell apoptotic process Source: BHF-UCL
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-12 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of immunoglobulin production Source: Ensembl
T cell costimulation Source: UniProtKB
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Cell membrane; Cell surface
CD40 ligand, soluble form: Secreted. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

Involvement in disease

Immunodeficiency with hyper-IgM, type 1 (HIGM1): Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.

Topology

Cytoplasmic: 1-22
Helical: 23-46
Extracellular: 47-261

PTM

The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Tang, T., Cheng, X., Truong, B., Sun, L., Yang, X., & Wang, H. (2021). Molecular basis and therapeutic implications of CD40/CD40L immune checkpoint. Pharmacology & Therapeutics, 219, 107709.

Bullock, T. N. (2021). CD40 stimulation as a molecular adjuvant for cancer vaccines and other immunotherapies. Cellular & Molecular Immunology, 1-9.

Dakal, T. C., Dhabhai, B., Agarwal, D., Gupta, R., Nagda, G., Meena, A. R., ... & Sharma, A. (2020). Mechanistic basis of co-stimulatory CD40-CD40L ligation mediated regulation of immune responses in cancer and autoimmune disorders. Immunobiology, 225(2), 151899.

Richards, D. M., Sefrin, J. P., Gieffers, C., Hill, O., & Merz, C. (2020). Concepts for agonistic targeting of CD40 in immuno-oncology. Human vaccines & immunotherapeutics, 16(2), 377-387.

Lai, J. H., Luo, S. F., & Ho, L. J. (2019). Targeting the CD40-CD154 signaling pathway for treatment of autoimmune arthritis. Cells, 8(8), 927.

Schlievert, P. M., Cahill, M. P., Hostager, B. S., Brosnahan, A. J., Klingelhutz, A. J., Gourronc, F. A., ... & Leung, D. Y. (2019). Staphylococcal superantigens stimulate epithelial cells through CD40 to produce chemokines. MBio, 10(2), e00214-19.

Foster, A. E., Mahendravada, A., Shinners, N. P., Chang, W. C., Crisostomo, J., Lu, A., ... & Spencer, D. M. (2017). Regulated expansion and survival of chimeric antigen receptor-modified T cells using small molecule-dependent inducible MyD88/CD40. Molecular Therapy, 25(9), 2176-2188.

Chen, J., Song, Y., Bojadzic, D., Tamayo-Garcia, A., Landin, A. M., Blomberg, B. B., & Buchwald, P. (2017). Small-molecule inhibitors of the CD40–CD40L costimulatory protein–protein interaction. Journal of medicinal chemistry, 60(21), 8906-8922.

Aarts, S. A., Seijkens, T. T., Kusters, P. J., van der Pol, S. M., Zarzycka, B., Heijnen, P. D., ... & Lutgens, E. (2017). Inhibition of CD40-TRAF6 interactions by the small molecule inhibitor 6877002 reduces neuroinflammation. Journal of neuroinflammation, 14(1), 1-14.

Chen, J., Li, J. H., Zhao, S. J., Wang, D. Y., Zhang, W. Z., & Liang, W. J. (2017). Clinical significance of costimulatory molecules CD40/CD40L and CD134/CD134L in coronary heart disease: a case-control study. Medicine, 96(32).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant CD40 protein (CAT#: V2LY-0526-LY1220)

Canine Recombinant CD40 protein, Fc Tag (CAT#: V2LY-0526-LY1221)

Canine Recombinant CD40 protein, hFc Tag (CAT#: V2LY-0526-LY1223)

Canine Recombinant CD40 protein, His Tag (CAT#: V2LY-0526-LY1224)

Human Recombinant CD40 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2861)

Human Recombinant CD40 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2862)

Human Recombinant CD40 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2863)

Human Recombinant CD40 protein, ECD, His Tag (CAT#: V2LY-0526-LY2864)

Human Recombinant CD40 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY2865)

Human Recombinant CD40 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY2866)

Antibody Pairs

CD40 Matched Antibody Pair (222) (CAT#: APMAB-222LY)

Related Products

Human Recombinant CD40 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY2865)

Human Recombinant CD40 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2861)

Armenian hamster Anti-CD40 Recombinant Antibody (HM40-3) (CAT#: CBMAB-C10365-LY)

CD40 Matched Antibody Pair (222) (CAT#: APMAB-222LY)

Rat Anti-CD40 Recombinant Antibody (CBFYC-1295) (CAT#: CBMAB-C1353-FY)

Canine Recombinant CD40 protein, His Tag (CAT#: V2LY-0526-LY1224)

Mouse Recombinant CD40 protein, ECD, His Tag (CAT#: V2LY-0526-LY8022)

Mouse Anti-CD40 Recombinant Antibody (CBFYC-1296) (CAT#: CBMAB-C1354-FY)

Rhesus Recombinant CD40 protein, hFc Tag (CAT#: V2LY-0526-LY9684)

Mouse Anti-CD40 Recombinant Antibody (CBFYC-1298) (CAT#: CBMAB-C1356-FY)

Isotype control

For research use only. Not intended for any clinical use.

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