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Human Cell Stress Antibody Array (Membrane, 26 Targets) (Y0246) (AbAr-0246-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Cell Stress Antibody Array detects 26 human cell stress-related proteins simultaneously.
Specifications
Target Array
Cell Stress Array
Target
ADAMTS1, HIF-2 alpha, Phospho-p38 alpha (T180/Y182), Bcl-2, Phospho-HSP27 (S78/S82), Phospho-p53 (S46), Carbonic Anhydrase IX, HSP60, PON1, Cited-2, HSP70, PON2, COX-2, IDO, PON3, Cytochrome c, Phospho-JNK Pan (T183/Y185), Thioredoxin-1, Dkk-4, NFkappaB1, SIRT2, FABP1/L-FABP, p21/CIP1, SOD2, HIF-1 alpha, p27/Kip1
Species Reactivity
Human
Sample Type
Cell, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Cellular stress response is the wide range of molecular changes that cells undergo in response to environmental stressors, including extremes of temperature, exposure to toxins, and mechanical damage.
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For Research Use Only. Not For Clinical Use.