Cynomolgus Recombinant ITGA5 beta 6 protein, ECD, His Tag (V2LY-0526-LY1585)

Name: Cynomolgus Recombinant ITGA5 beta 6 protein, ECD, His Tag
SKU: V2LY-0526-LY1585
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

His Tag

Protein Construction

This product is Cynomolgus Recombinant ITGA5 beta 6 protein, ECD, His Tag consist of Amino Acid: 31-993, 22-709 and predicts a molecular mass of 192.54 kDa.

Molecule Mass

192.54 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 31-993, 22-709

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile CAN, TFA

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Integrin Subunit Alpha 5

Function

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).
ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).
(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.
(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.
(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Biological Process

AngiogenesisManual Assertion Based On ExperimentTAS:BHF-UCL
CD40 signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Cell adhesion mediated by integrinManual Assertion Based On ExperimentIDA:UniProtKB
Cell-cell adhesion mediated by integrinIEA:Ensembl
Cell-substrate adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Cell-substrate junction assemblyIEA:Ensembl
Endodermal cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Female pregnancyIEA:Ensembl
Heterophilic cell-cell adhesion via plasma membrane cell adhesion moleculesIEA:Ensembl
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Leukocyte cell-cell adhesionIEA:Ensembl
MemoryIEA:Ensembl
Negative regulation of anoikisManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of cell migrationIEA:Ensembl
Positive regulation of cell-substrate adhesionIEA:Ensembl
Positive regulation of peptidyl-tyrosine phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of sprouting angiogenesisManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of vascular endothelial growth factor receptor signaling pathwayManual Assertion Based On ExperimentTAS:BHF-UCL
Wound healing, spreading of epidermal cellsManual Assertion Based On ExperimentIEP:BHF-UCL

Cellular Location

Membrane; Cell junction, focal adhesion; Cell surface

Topology

Extracellular: 42-995
Helical: 996-1021
Cytoplasmic: 1022-1049

PTM

Proteolytic cleavage by PCSK5 mediates activation of the precursor.

Wang, J. F., Chen, Y. Y., Zhang, S. W., Zhao, K., Qiu, Y., Wang, Y., ... & Chen, J. Q. (2022). ITGA5 promotes tumor progression through the activation of the FAK/AKT signaling pathway in human gastric cancer. Oxidative Medicine and Cellular Longevity, 2022.

Zhou, C., Shen, Y., Wei, Z., Shen, Z., Tang, M., Shen, Y., & Deng, H. (2022). ITGA5 is an independent prognostic biomarker and potential therapeutic target for laryngeal squamous cell carcinoma. Journal of Clinical Laboratory Analysis, 36(2), e24228.

Assidicky, R., Tokat, U. M., Tarman, I. O., Saatci, O., Ersan, P. G., Raza, U., ... & Sahin, O. (2022). Targeting HIF1-alpha/miR-326/ITGA5 axis potentiates chemotherapy response in triple-negative breast cancer. Breast cancer research and treatment, 193(2), 331-348.

Zhu, H., Wang, G., Zhu, H., & Xu, A. (2021). ITGA5 is a prognostic biomarker and correlated with immune infiltration in gastrointestinal tumors. BMC cancer, 21, 1-14.

Jiang, Z., Wei, J., Yang, W., Li, W., Liu, F., Yan, X., ... & Li, J. (2020). MicroRNA-26a inhibits wound healing through decreased keratinocytes migration by regulating ITGA5 through PI3K/AKT signaling pathway. Bioscience Reports, 40(9), BSR20201361.

Feng, C., Jin, X., Han, Y., Guo, R., Zou, J., Li, Y., & Wang, Y. (2020). Expression and prognostic analyses of ITGA3, ITGA5, and ITGA6 in head and neck squamous cell carcinoma. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 26, e926800-1.

Yan, T., & Ye, X. X. (2019). MicroRNA-328-3p inhibits the tumorigenesis of bladder cancer through targeting ITGA5 and inactivating PI3K/AKT pathway. European Review for Medical & Pharmacological Sciences, 23(12).

Deng, Y., Wan, Q., & Yan, W. (2019). Integrin α5/ITGA5 promotes the proliferation, migration, invasion and progression of oral squamous carcinoma by epithelial–mesenchymal transition. Cancer management and research, 9609-9620.

Yu, M., Chu, S., Fei, B., Fang, X., & Liu, Z. (2019). O-GlcNAcylation of ITGA5 facilitates the occurrence and development of colorectal cancer. Experimental Cell Research, 382(2), 111464.

Kuninty, P. R., Bansal, R., De Geus, S. W., Mardhian, D. F., Schnittert, J., van Baarlen, J., ... & Prakash, J. (2019). ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer. Science advances, 5(9), eaax2770.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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