Mouse Anti-DsbA-tag Recombinant Antibody (V2-610553) (CBMAB-Z0005-LY)

Name: Mouse Anti-DsbA-tag Recombinant Antibody (V2-610553)
SKU: CBMAB-Z0005-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

V2-610553

Application

Immunogen

Purified recombinant E.coli. DsbA protein fragments over expressed in E.coli.

Specificity

E. coli

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 0.1 mg/ml BSA and 50% glycerol.

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DsbA-tag

Entrez Gene ID

8114936

UniProt ID

P0AEG4

Alternative Names

DSBA like thioredoxin domain containing protein.

Research Area

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.

Biological Process

Cellular response to antibiotic Source: EcoliWiki

Cellular Location

Periplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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