Anti-ESR1 Antibody (CBMAB-1213-YC) Immunohistochemistry Kit (V2LY-0826-LY174)

Name: Anti-ESR1 Antibody (CBMAB-1213-YC) Immunohistochemistry Kit
SKU: V2LY-0826-LY174
Rating: 5 (1 reviews)

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Summary

Product Overview

This comprehensive, all-in-one kit is designed for the precise immunohistochemical (IHC) detection of target protein in tissue samples. Unlike standard detection kits, it includes a highly specific rabbit primary antibody bundled directly with our robust Polymer HRP detection system. The kit provides every essential reagent needed for the entire IHC workflow-from the initial antigen retrieval buffer to the final mounting media. By offering a pre-matched primary antibody with optimized buffers and secondary detection reagents, this kit eliminates the need for tedious protocol optimization and reagent sourcing, ensuring consistent, high-quality staining results.

Primary Antibody Cat.

CBMAB-1213-YC

Primary Antibody

Rabbit Anti-ESR1 Recombinant Antibody (E91)

Secondary Antibody Host Species

Goat

Secondary Antibody Species Specificity

Rabbit

Application

IHC-P

Sample Type

FFPE tissue

Test Size

50 Tests

Storage

Stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Key Features

Target-Specific Primary Antibody

Includes a highly validated rabbit primary antibody, ensuring reliable and specific target localization.

Complete End-to-End Solution

Contains all 11 essential components required for a full IHC workflow, including Tris-EDTA Antigen Retrieval Buffer, Blocking Buffer, Polymer HRP-Goat anti-Rabbit Secondary Antibody, DAB Chromogens, Counterstain, and Mounting Media.

Streamlined Workflow

Pre-optimized reagents work seamlessly with the included primary antibody, saving researchers significant time on antibody titration and protocol adjustments.

Sample Compatibility

Highly recommended and optimized for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

Kit Capacity

Provides sufficient reagents (50T) to perform 50 tests, depending on tissue section size.

More Infomation

Sub Cat	Reagents	Size
LV01	Antigen Retrieval Buffer (50× Tris-EDTA)	100 ml
LV02	Washing Buffer (30×)	120 ml
LV03	Blocking Buffer	5 ml
LV04	Quenching buffer (RTU)	5 ml
LV05	Primary Antibody Diluent (RTU)	100 ml
LV06R	HRP-Goat anti-Rabbit Secondary Antibody	5 ml
LV07	Chromogen Component A	0.2 ml
LV08	Chromogen Component B	4 ml
LV09	Signal Enhancer	5 ml
LV10	Counter Staining Reagent	5 ml
LV11	Mounting Media	5 ml

Liang, J., Ingalla, E. R., Yao, X., Wang, B. E., Tai, L., Giltnane, J., ... & Metcalfe, C. (2022). Giredestrant reverses progesterone hypersensitivity driven by estrogen receptor mutations in breast cancer. Science Translational Medicine, 14(663), eabo5959.

Pelusi, L., Mandatori, D., Di Pietrantonio, N., Del Pizzo, F., Di Tomo, P., Di Pietro, N., ... & Pipino, C. (2022). Estrogen Receptor 1 (ESR1) and the Wnt/β-Catenin Pathway Mediate the Effect of the Coumarin Derivative Umbelliferon on Bone Mineralization. Nutrients, 14(15), 3209.

Gregorio, K. C. R., Laurindo, C. P., & Machado, U. F. (2021). Estrogen and glycemic homeostasis: the fundamental role of nuclear estrogen receptors ESR1/ESR2 in glucose transporter GLUT4 regulation. Cells, 10(1), 99.

Tan, S. C., Low, T. Y., Mohamad Hanif, E. A., Sharzehan, M. A. K., Kord-Varkaneh, H., & Islam, M. A. (2021). The rs9340799 polymorphism of the estrogen receptor alpha (ESR1) gene and its association with breast cancer susceptibility. Scientific reports, 11(1), 1-12.

Ulhaq, Z. S. (2020). Update on “associations of estrogen receptor alpha gene polymorphisms with type 2 diabetes mellitus and metabolic syndrome: a systematic review and meta-analysis”. Hormone and Metabolic Research, 52(01), 67-70.

Al-Amri, R. J., Alotibi, M. K. H., Al-Raddadi, R. I., Shebli, W. T. Y., Fallatah, E. I. Y., Alhujaily, A. S., & Mohamed, H. S. (2020). Estrogen receptor 1 gene (ESR1) rs2234693 polymorphism and breast cancer risk in Saudi women. Asian Pacific Journal of Cancer Prevention: APJCP, 21(11), 3235.

Toyota, K., Masuda, S., Sugita, S., Miyaoku, K., Yamagishi, G., Akashi, H., & Miyagawa, S. (2020). Estrogen Receptor 1 (ESR1) Agonist Induces Ovarian Differentiation and Aberrant Müllerian Duct Development in the Chinese Soft-Shelled Turtle, Pelodiscus Sinensi. Zoological studies, 59.

Lei, J. T., Gou, X., Seker, S., & Ellis, M. J. (2019). ESR1 alterations and metastasis in estrogen receptor positive breast cancer. Journal of cancer metastasis and treatment, 5.

Hodgkinson, K., Forrest, L. A., Vuong, N., Garson, K., Djordjevic, B., & Vanderhyden, B. C. (2018). GREB1 is an estrogen receptor-regulated tumour promoter that is frequently expressed in ovarian cancer. Oncogene, 37(44), 5873-5886.

Barreto-Andrade, J. N., de Fátima, L. A., Campello, R. S., Guedes, J. A. C., de Freitas, H. S., & Machado, M. M. O. U. F. (2018). Estrogen receptor 1 (ESR1) enhances Slc2a4/GLUT4 expression by a SP1 cooperative mechanism. International Journal of Medical Sciences, 15(12), 1320.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Esr1 ELISA Kit (CAT#: V2LY-0626-LY1693)

Human ESR1 ELISA Kit (CAT#: V2LY-0626-LY5784)

Anti-ESR1 Antibody (CBMAB-1208-YC) Immunohistochemistry Kit (CAT#: V2LY-0826-LY172)

Anti-ESR1 Antibody (CBMAB-1212-YC) Immunohistochemistry Kit (CAT#: V2LY-0826-LY173)

Anti-ESR1 Antibody (CBMAB-1214-YC) Immunohistochemistry Kit (CAT#: V2LY-0826-LY175)

Anti-ESR1 Antibody (CBMAB-E1865-FY) Immunohistochemistry Kit (CAT#: V2LY-0826-LY176)

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Rabbit Anti-ESR1 Recombinant Antibody (E91) (CAT#: CBMAB-1213-YC)

Human ESR1 ELISA Kit (CAT#: V2LY-0626-LY5784)

Mouse Anti-ESR1 Recombinant Antibody (CBT4734) (CAT#: V2LY-0625-LY2277)

Mouse Anti-ESR1 Recombinant Antibody (
2C10) (CAT#: V2LY-0725-LY399)

Rabbit Anti-ESR1 Recombinant Antibody (1739) (CAT#: V2LY-0825-LY781)

Mouse Anti-ESR1 Recombinant Antibody (CBT514) (CAT#: V2LY-0625-LY3458)

Anti-ESR1 Antibody (CBMAB-E1865-FY) Immunohistochemistry Kit (CAT#: V2LY-0826-LY176)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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