Hamster Recombinant FUT8 protein, His Tag (V2LY-0526-LY1811)

Name: Hamster Recombinant FUT8 protein, His Tag
SKU: V2LY-0526-LY1811
Rating: 5 (1 reviews)

See all FUT8 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Hamster

Tag

His Tag

Protein Construction

This product is Hamster Recombinant FUT8 protein, His Tag consist of Amino Acid: 68-575 and predicts a molecular mass of 60.1 kDa.

Molecule Mass

60.1 kDa

Sequence

Amino Acid: 68-575

Species

Hamster

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Fucosyltransferase 8

Function

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.

Biological Process

Cell migration Source: Ensembl
GDP-L-fucose metabolic process Source: UniProtKB
Integrin-mediated signaling pathway Source: Ensembl
In utero embryonic development Source: UniProtKB
L-fucose catabolic process Source: UniProtKB
N-glycan fucosylation Source: GO_Central
N-glycan processing Source: UniProtKB
Oligosaccharide biosynthetic process Source: ProtInc
Protein glycosylation in Golgi Source: InterPro
Protein N-linked glycosylation Source: GO_Central
Protein N-linked glycosylation via asparagine Source: UniProtKB
Receptor metabolic process Source: Ensembl
Regulation of cellular response to oxidative stress Source: Ensembl
Regulation of gene expression Source: Ensembl
Respiratory gaseous exchange by respiratory system Source: Ensembl
Transforming growth factor beta receptor signaling pathway Source: Ensembl
Viral protein processing Source: Reactome

Cellular Location

Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.

Involvement in disease

Congenital disorder of glycosylation with defective fucosylation 1 (CDGF1):
A form of congenital disorder of glycosylation, a genetically heterogeneous group of multisystem disorders caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. CDGF1 is an autosomal recessive disorder, apparent from birth, characterized by poor growth, failure to thrive, hypotonia, skeletal anomalies, and delayed psychomotor development with intellectual disability.

Topology

Cytoplasmic: 1-9
Helical: 10-30
Lumenal: 31-575

PTM

Tyrosine phosphorylated by PKDCC/VLK.

Kyunai, Y. M., Sakamoto, M., Koreishi, M., Tsujino, Y., & Satoh, A. (2023). Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response. Plos one, 18(2), e0281516.

Cuvry, A., Gozalbo-Rovira, R., Strubbe, D., Neyts, J., de Witte, P., Rodríguez-Díaz, J., & Rocha-Pereira, J. (2022). The Role of Histo-Blood Group Antigens and Microbiota in Human Norovirus Replication in Zebrafish Larvae. Microbiology Spectrum, 10(6), e03157-22.

Liang, Y., Wang, T., Gao, R., Jia, X., Ji, T., Shi, P., ... & Han, P. (2022). Fucosyltransferase 8 is Overexpressed and Influences Clinical Outcomes in Lung Adenocarcinoma Patients. Pathology and Oncology Research, 9.

Bastian, K., Scott, E., Elliott, D. J., & Munkley, J. (2021). FUT8 alpha-(1, 6)-fucosyltransferase in cancer. International Journal of Molecular Sciences, 22(1), 455.

Ma, M., Guo, D., Tan, Z., Du, J., Guan, F., & Li, X. (2021). Fucosyltransferase 8 regulation and breast cancer suppression by transcription factor activator protein 2γ. Cancer Science, 112(8), 3190-3204.

Boruah, B. M., Kadirvelraj, R., Liu, L., Ramiah, A., Li, C., Zong, G., ... & Moremen, K. W. (2020). Characterizing human α-1, 6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases. Journal of Biological Chemistry, 295(50), 17027-17045.

Tada, K., Ohta, M., Hidano, S., Watanabe, K., Hirashita, T., Oshima, Y., ... & Inomata, M. (2020). Fucosyltransferase 8 plays a crucial role in the invasion and metastasis of pancreatic ductal adenocarcinoma. Surgery Today, 50, 767-777.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Hamster Recombinant FUT8 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant FUT8 protein, His Tag (CAT#: V2LY-0526-LY4223)

Related Products

Human Recombinant FUT8 protein, His Tag (CAT#: V2LY-0526-LY4223)

Mouse Anti-FUT8 Recombinant Antibody (CF176) (CAT#: CBMAB-FT310LY)

Rabbit Anti-FUT8 Recombinant Antibody (CBXF-1118) (CAT#: CBMAB-F0996-CQ)

Mouse Anti-FUT8 Recombinant Antibody (980) (CAT#: CBMAB-AP2419LY)

Mouse Anti-FUT8 Monoclonal Antibody (Y41C) (CAT#: CBMAB-1291-YC)

Mouse Anti-FUT8 Recombinant Antibody (CBXF-2541) (CAT#: CBMAB-F1152-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry