Human CENPK ELISA Kit (V2LY-0626-LY4394)

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Tested Data
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Basic Information

Sensitivity
0.007 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
31.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Centromere Protein K
Function
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
Biological Process
CENP-A containing nucleosome assembly Source: Reactome
Kinetochore assembly Source: InterPro
Mitotic sister chromatid segregation Source: GO_Central
Mitotic spindle organization Source: Reactome
Cellular Location
Nucleus; Centromere; Kinetochore. Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
Involvement in disease
Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.

Li, Q., Liang, J., Zhang, S., An, N., Xu, L., & Ye, C. (2021). Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration. Bioengineered, 12(1), 1299-1310.

Wang, Y., Ren, C., Wang, H., Zhang, Y., & Xiu, Y. (2021). Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion. Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University.

Ma, J., Chen, X., Lin, M., Wang, Z., Wu, Y., & Li, J. (2021). Bioinformatics analysis combined with experiments predicts CENPK as a potential prognostic factor for lung adenocarcinoma. Cancer cell international, 21(1), 1-14.

Wu, S., Cao, L., Ke, L., Yan, Y., Luo, H., Hu, X., ... & He, Y. (2021). Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN‐PI3K‐AKT signalling pathway in gastric cancer. Journal of Cellular and Molecular Medicine, 25(18), 8890-8903.

Xie, F., Yuan, B., & Liu, J. (2021). Downregulation of CENPK suppresses lung adenocarcinoma by regulating EMT. Archives of Medical Science.

Wang, J., Li, H., Xia, C., Yang, X., Dai, B., Tao, K., & Dou, K. (2019). Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1. OncoTargets and therapy, 12, 869.

Wang, H., Liu, W., Liu, L., Wu, C., Wu, W., Zheng, J., ... & Huang, J. (2017). Overexpression of centromere protein K (CENP-K) gene in hepatocellular carcinoma promote cell proliferation by activating AKT/TP53 signal pathway. Oncotarget, 8(43), 73994.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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