Human CLSTN1 ELISA Kit (V2LY-0626-LY5775)

Name: Human CLSTN1 ELISA Kit
SKU: V2LY-0626-LY5775
Rating: 5 (1 reviews)

See all CLSTN1 antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.012 ng/mL

Detection Range

0.02-3.8 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

109.8 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Calsyntenin 1

Function

Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals (By similarity).

Biological Process

Cell adhesion Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
Neurotransmitter receptor transport to postsynaptic membrane Source: Ensembl
Positive regulation of synapse assembly Source: GO_Central
Positive regulation of synaptic transmission Source: GO_Central
Regulation of cell growth Source: Ensembl
Regulation of synapse maturation Source: Ensembl
Vesicle-mediated transport in synapse Source: Ensembl

Cellular Location

Golgi apparatus membrane; Postsynaptic cell membrane; Endoplasmic reticulum membrane; Nucleus; Neuron projection. Neurite tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1.

Topology

Extracellular: 29-859
Helical: 860-880
Cytoplasmic: 881-981

PTM

Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1.

Lee, E. S., Kim, W. T., Park, G. Y., Lee, M., & Gen Son, T. (2021). Calsyntenin 1 mRNA expression sensitivity to ionizing radiation in human hepatocytes and carcinoma cells and blood cells of BALB/c mice. Journal of Radiation Research and Applied Sciences, 14(1), 44-50.

Ou, M. Y., Xiao, Q., Ju, X. C., Zeng, P. M., Huang, J., Sheng, A. L., & Luo, Z. G. (2021). The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development. Cell Reports, 35(13), 109290.

Chiou, J., & Hsiao, M. (2020). Repurposing Calsyntenin‐1 as a modulator in FSTL1‐mediated Lung Adenocarcinoma Metastasis: The Crosstalk with extracellular ALDOA. The FASEB Journal, 34(S1), 1-1.

Cheng, K., Chen, Y. S., Yue, C. X., Zhang, S. M., Pei, Y. P., Cheng, G. R., ... & Zeng, Y. (2019). Calsyntenin-1 negatively regulates ICAM5 accumulation in postsynaptic membrane and influences dendritic spine maturation in a mouse model of fragile X syndrome. Frontiers in neuroscience, 13, 1098.

Lee, T. J., Lee, J. W., Haynes, E. M., Eliceiri, K. W., & Halloran, M. C. (2017). The kinesin adaptor calsyntenin-1 organizes microtubule polarity and regulates dynamics during sensory axon arbor development. Frontiers in cellular neuroscience, 11, 107.

Chu, Y., Lai, Y. H., Lee, M. C., Yeh, Y. J., Wu, Y. K., Tsao, W., ... & Wu, S. (2017). Calsyntenin-1, clusterin and neutrophil gelatinase-associated lipocalin are candidate serological biomarkers for lung adenocarcinoma. Oncotarget, 8(64), 107964.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human CLSTN1 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CLSTN1 protein, ECD, His Tag (CAT#: V2LY-0526-LY3245)

Related Products

Rabbit Anti-CLSTN1 Recombinant Antibody (1388) (CAT#: V2LY-1206-LY296)

Human Recombinant CLSTN1 protein, ECD, His Tag (CAT#: V2LY-0526-LY3245)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry