Human coronavirus Recombinant (HCoV-NL63) Spike S1+S2 protein, ECD, Biotin Conjugated, His Tag (V2LY-0526-LY167)

Name: Human coronavirus Recombinant (HCoV-NL63) Spike S1+S2 protein, ECD, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY167
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human coronavirus

Tag

His Tag

Protein Construction

This product is Human coronavirus Recombinant (HCoV-NL63) Spike S1+S2 protein, ECD, Biotin Conjugated, His Tag consist of Amino Acid: 1-1296 and predicts a molecular mass of 142.4 kDa.

Molecule Mass

142.4 kDa

Protein Domain

ECD

Conjugates

Biotin

Sequence

Amino Acid: 1-1296

Species

Human coronavirus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>75% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Spike glycoprotein

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

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Ebola virus Recombinant (subtype Zaire, strain Mayinga 1976) GP-RBD protein (CAT#: V2LY-0526-LY89)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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