Human ADAMTS5 ELISA Kit (V2LY-0626-LY4305)

Name: Human ADAMTS5 ELISA Kit
SKU: V2LY-0626-LY4305
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

4.69 ng/mL

Detection Range

10-2000 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

101.7 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

ADAM Metallopeptidase With Thrombospondin Type 1 Motif 5

Function

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.

Biological Process

Defense response to bacterium
Extracellular matrix disassembly
Extracellular matrix organization
Myoblast fusion
Negative regulation of cold-induced thermogenesis
Proteolysis
Tooth eruption

Cellular Location

Extracellular matrix

PTM

The precursor is cleaved by furin and PCSK7 outside of the cell.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).

Wang, W., Zhang, H., Hou, C., Liu, Q., Yang, S., Zhang, Z., ... & Yang, X. (2021). Internal modulation of proteolysis in vascular extracellular matrix remodeling: role of ADAM metallopeptidase with thrombospondin type 1 motif 5 in the development of intracranial aneurysm rupture. Aging (Albany NY), 13(9), 12800.

Weng, K., Luo, M., & Dong, D. (2020). Elucidation of the Mechanism by Which a ADAMTS5 Gene MicroRNA-Binding Site Single Nucleotide Polymorphism Affects the Risk of Osteoarthritis. Genetic Testing and Molecular Biomarkers, 24(8), 467-477.

Zeng, T., Gan, J., Liu, Y., Shi, L., Lu, Z., Xue, Y., ... & Yuan, J. (2020). ADAMTS-5 decreases in aortas and plasma from aortic dissection patients and alleviates angiotensin II-induced smooth muscle-cell apoptosis. Frontiers in Cardiovascular Medicine, 7.

Ozler, S., Oztas, E., Gumus Guler, B., Erel, O., Turhan Caglar, A., Ergin, M., ... & Danisman, N. (2020). Are serum levels of ADAMTS5, TAS and TOS at 24–28 gestational weeks associated with adverse perinatal outcomes in gestational diabetic women?. Journal of Obstetrics and Gynaecology, 40(5), 619-625.

Wang, Z., Ye, D., Ye, J., Wang, M., Liu, J., Jiang, H., ... & Wan, J. (2019). ADAMTS-5 decreases in coronary arteries and plasma from patients with coronary artery disease. Disease markers, 2019.

Fava, M., Barallobre-Barreiro, J., Mayr, U., Lu, R., Didangelos, A., Baig, F., ... & Mayr, M. (2018). Role of ADAMTS-5 in aortic dilatation and extracellular matrix remodeling. Arteriosclerosis, thrombosis, and vascular biology, 38(7), 1537-1548.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Adamts5 ELISA Kit (CAT#: V2LY-0626-LY1789)

Cattle ADAMTS5 ELISA Kit (CAT#: V2LY-0626-LY2473)

Related Products

Cattle ADAMTS5 ELISA Kit (CAT#: V2LY-0626-LY2473)

Mouse Adamts5 ELISA Kit (CAT#: V2LY-0626-LY1789)

Mouse Anti-ADAMTS5 Recombinant Antibody (V2-179852) (CAT#: CBMAB-A1217-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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