Human DEGS1 ELISA Kit (V2LY-0626-LY5277)

Name: Human DEGS1 ELISA Kit
SKU: V2LY-0626-LY5277
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.003 ng/mL

Detection Range

0.007-1.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

37.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

degenerative spermatocyte homolog 1, lipid desaturase (Drosophila)

Function

Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine) (PubMed:11937514, PubMed:30620337, PubMed:30620338).

Catalyzes the equilibrium isomerization of retinols (By similarity).

Biological Process

Ceramide biosynthetic process Source: GO_Central
Myelin maintenance Source: UniProtKB
Neutrophil degranulation Source: Reactome
Dphingolipid biosynthetic process Source: Reactome
Unsaturated fatty acid biosynthetic process Source: ProtInc

Cellular Location

Endoplasmic reticulum membrane; Mitochondrion membrane

Involvement in disease

Leukodystrophy, hypomyelinating, 18 (HLD18):
An autosomal recessive disorder characterized by hypomyelinating leukodystrophy with progressive atrophy of the corpus callosum, thalami and cerebellum, and peripheral neuropathy. Clinical features include very poor psychomotor development, dystonia, severe spasticity, seizures, and failure to thrive.

PTM

Myristoylation can target the enzyme to the mitochondria leading to an increase in ceramide levels.

Yi, L., Liu, J., Deng, M., Zuo, H., & Li, M. (2021). Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis. BMC pulmonary medicine, 21(1), 1-16.

Jin, S., Lee, S. H., Lee, D. H., Manjula, P., Lee, S. H., & Lee, J. H. (2020). Genetic association of DEGS1, ELOVL6, FABP3, FABP4, FASN and SCD genes with fatty acid composition in breast and thigh muscles of Korean native chicken. Anim Genet, 51(2), 344-345.

Pant, D. C., Dorboz, I., Schluter, A., Fourcade, S., Launay, N., Joya, J., ... & Pujol, A. (2019). Loss of the sphingolipid desaturase DEGS1 causes hypomyelinating leukodystrophy. The Journal of clinical investigation, 129(3), 1240-1256.

Blackburn, N. B., Michael, L. F., Meikle, P. J., Peralta, J. M., Mosior, M., McAhren, S., ... & Curran, J. E. (2019). Rare DEGS1 variant significantly alters de novo ceramide synthesis pathway [S]. Journal of lipid research, 60(9), 1630-1639.

Dolgin, V., Straussberg, R., Xu, R., Mileva, I., Yogev, Y., Khoury, R., ... & Birk, O. S. (2019). DEGS1 variant causes neurological disorder. European Journal of Human Genetics, 27(11), 1668-1676.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rabbit Anti-DEGS1 Recombinant Antibody (D0695) (CAT#: CBMAB-D0695-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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