Human GOLGA5 ELISA Kit (V2LY-0626-LY3399)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0025 ng/mL
Detection Range
0.005-1 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
83.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Golgin A5
Function
Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
Biological Process
Golgi organization Source: UniProtKB
Golgi vesicle transport Source: UniProtKB
Retrograde transport, vesicle recycling within Golgi Source: GO_Central
Cellular Location
Golgi apparatus membrane. Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network.
Involvement in disease
A chromosomal aberration involving GOLGA5 is found in papillary thyroid carcinomas (PTCs). Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene.
Topology
Cytoplasmic: 2-698
Helical: 699-719
Lumenal: 720-731
PTM
Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.

Asensio, C. S. (2020). 253-LB: A Novel Membrane Binding Domain in Conjunction with Golga5 Recruits the Secretory Granule Biogenesis Factor HID-1 to the Golgi. Diabetes, 69(Supplement_1).

Ding, Y. Y., Stern, J. W., Jubelirer, T. F., Wertheim, G. B., Lin, F., Chang, F., ... & Tasian, S. K. (2018). Clinical efficacy of ruxolitinib and chemotherapy in a child with Philadelphia chromosome-like acute lymphoblastic leukemia with GOLGA5-JAK2 fusion and induction failure. Haematologica, 103(9), e427-e431.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

GOLGA5 Matched Antibody Pair (492) (CAT#: APMAB-492LY)

Antibody Pairs

GOLGA5 Matched Antibody Pair (492) (CAT#: APMAB-492LY)

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Mouse Anti-GOLGA5 Recombinant Antibody (CAP848) (CAT#: CBMAB-AP2576LY)

Mouse Anti-GOLGA5 Recombinant Antibody (CBFYH-0412) (CAT#: V2LY-0226-LY297)

GOLGA5 Matched Antibody Pair (492) (CAT#: APMAB-492LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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