Human METAP2 ELISA Kit (2) (V2LY-0626-LY4019)

Name: Human METAP2 ELISA Kit (2)
SKU: V2LY-0626-LY4019
Rating: 5 (1 reviews)

See all METAP2 antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0075 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

52.9 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Methionyl Aminopeptidase 2

Function

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.

Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

Biological Process

N-terminal protein amino acid modification Source: HGNC-UCL
Peptidyl-methionine modification Source: HGNC-UCL
Protein initiator methionine removal Source: UniProtKB-UniRule
Protein processing Source: HGNC-UCL

Cellular Location

Cytoplasm
Note: About 30% of expressed METAP2 associates with polysomes.

PTM

Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.

Zhang, K., Hu, J., & Zhao, Z. (2023). Fumagillin regulates stemness and malignancies in cancer stem-like cells derived from liver cancer via targeting to MetAP-2. Plos one, 18(7), e0289024.

Birgül, K., Uba, A. I., Çuhadar, O., Sevinç, S. K., Tiryaki, S., Tiber, P. M., ... & Küçükgüzel, Ş. G. (2022). Synthesis and molecular modeling of MetAP2 of thiosemicarbazides, 1, 2, 4-triazoles, thioethers derived from (S)-Naproxen as possible breast cancer agents. Journal of Molecular Structure, 1259, 132739.

Demers, M., Sturtevant, S., Guertin, K. R., Gupta, D., Desai, K., Vieira, B. F., ... & Light, D. R. (2021). MetAP2 inhibition modifies hemoglobin S to delay polymerization and improves blood flow in sickle cell disease. Blood Advances, 5(5), 1388-1402.

Shaikh, S. K. J., Kamble, R. R., Bayannavar, P. K., Somagond, S. M., & Joshi, S. D. (2020). Triazolothiadizepinylquinolines as potential MetAP-2 and NMT inhibitors: Microwave-assisted synthesis, pharmacological evaluation and molecular docking studies. Journal of Molecular Structure, 1203, 127445.

He, W. P., Guo, Y. Y., Yang, G. P., Lai, H. L., Sun, T. T., Zhang, Z. W., ... & Yang, G. F. (2020). CHD1L promotes EOC cell invasiveness and metastasis via the regulation of METAP2. International Journal of Medical Sciences, 17(15), 2387.

Siddik, M. A. B., Das, B. C., Weiss, L., Dhurandhar, N. V., & Hegde, V. (2019). A MetAP2 inhibitor blocks adipogenesis, yet improves glucose uptake in cells. Adipocyte, 8(1), 240-253.

Heinrich, T., Seenisamy, J., Becker, F., Blume, B., Bomke, J., Dietz, M., ... & Zenke, F. T. (2019). Identification of methionine aminopeptidase-2 (MetAP-2) inhibitor M8891: A clinical compound for the treatment of cancer. Journal of Medicinal Chemistry, 62(24), 11119-11134.

Heinrich, T., Seenisamy, J., Blume, B., Bomke, J., Calderini, M., Eckert, U., ... & Zenke, F. T. (2019). Discovery and structure-based optimization of next-generation reversible methionine aminopeptidase-2 (MetAP-2) inhibitors. Journal of medicinal chemistry, 62(10), 5025-5039.

Farrell, P. J., Zopf, C. J., Huang, H. J., Balakrishna, D., Holub, C., Bilakovics, J., ... & Larson, C. J. (2019). Using target engagement biomarkers to predict clinical efficacy of MetAP2 inhibitors. Journal of Pharmacology and Experimental Therapeutics, 371(2), 299-308.

Huang, H. J., Holub, C., Rolzin, P., Bilakovics, J., Fanjul, A., Satomi, Y., ... & Farrell, P. J. (2019). MetAP2 inhibition increases energy expenditure through direct action on brown adipocytes. Journal of Biological Chemistry, 294(24), 9567-9575.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human METAP2 ELISA Kit (2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant METAP2 protein, His Tag (CAT#: V2LY-0526-LY5560)

Mouse Recombinant METAP2 protein, His Tag (CAT#: V2LY-0526-LY8671)

Human METAP2 ELISA Kit (CAT#: V2LY-0626-LY2917)

Related Products

Human Recombinant METAP2 protein, His Tag (CAT#: V2LY-0526-LY5560)

Human METAP2 ELISA Kit (CAT#: V2LY-0626-LY2917)

Mouse Recombinant METAP2 protein, His Tag (CAT#: V2LY-0526-LY8671)

Rabbit Anti-METAP2 Recombinant Antibody (D3I1H) (CAT#: CBMAB-CP1522-LY)

Rabbit Anti-METAP2 Recombinant Antibody (
8F2) (CAT#: V2LY-0725-LY1179)

Mouse Anti-METAP2 (AA 2-212) Recombinant Antibody (CBFYM-2112) (CAT#: CBMAB-M2294-FY)

Rabbit Anti-METAP2 Recombinant Antibody (CBFYM-2113) (CAT#: CBMAB-M2295-FY)

Rabbit Anti-METAP2 Recombinant Antibody (CBFYM-2114) (CAT#: CBMAB-M2296-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry