Human MLPH ELISA Kit (V2LY-0626-LY4036)

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Tested Data
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Basic Information

Sensitivity
0.0052 ng/mL
Detection Range
0.01-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
65.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Melanophilin
Function
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
Biological Process
Intracellular protein transport Source: InterPro
Melanosome transport Source: UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Griscelli syndrome 3 (GS3):
Rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations.

Kim, J. Y., Han, S. Y., Sung, K., Seo, J. Y., Myung, C. H., Jo, C. S., ... & Hwang, J. S. (2023). Inhibition of Melanosome Transport by Inducing Exon Skipping in Melanophilin. Biomolecules & Therapeutics, 31(4), 466.

Pan, J., Zhou, R., Yao, L. L., Zhang, J., Zhang, N., Cao, Q. J., ... & Li, X. D. (2023). Melanophilin mediates the association of myosin-5a with melanosome via three distinct interactions. bioRxiv, 2023-11.

Yuan, Z., Zhang, X., Pang, Y., Qi, Y., Wang, Q., Hu, Y., ... & Huo, L. (2023). Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail. Archives Animal Breeding, 66(1), 131-139.

Myung, C. H., Lee, J. E., Jo, C. S., Park, J. I., & Hwang, J. S. (2021). Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR). Scientific Reports, 11(1), 16813.

Ermini, L., Francis, J. C., Rosa, G. S., Rose, A. J., Ning, J., Greaves, M., & Swain, A. (2021). Evolutionary selection of alleles in the melanophilin gene that impacts on prostate organ function and cancer risk. Evolution, Medicine, and Public Health, 9(1), 311-321.

Li, J., Chen, Y., Liu, M., Chen, Q., Zhou, J., Bao, G., & Wu, X. (2020). Association of Melanophilin (MLPH) gene polymorphism with coat colour in Rex rabbits. World Rabbit Science, 28(1), 29-38.

Cao, Q. J., Zhang, N., Zhou, R., Yao, L. L., & Li, X. D. (2019). The cargo adaptor proteins RILPL2 and melanophilin co-regulate myosin-5a motor activity. Journal of Biological Chemistry, 294(29), 11333-11341.

Robinson, C. L., Evans, R. D., Sivarasa, K., Ramalho, J. S., Briggs, D. A., & Hume, A. N. (2019). The adaptor protein melanophilin regulates dynamic myosin-Va: cargo interaction and dendrite development in melanocytes. Molecular Biology of the Cell, 30(6), 742-752.

Park, J. I., Lee, J. E., Myung, C. H., Jo, C. S., Jang, H. S., & Hwang, J. S. (2019). The absence of Rab27a accelerates the degradation of Melanophilin. Experimental Dermatology, 28(1), 90-93.

Chung, C. T., Marrano, P., Swanson, D., Dickson, B. C., & Thorner, P. S. (2019). Fusion of ALK to the melanophilin gene MLPH in pediatric Spitz nevi. Human Pathology, 87, 57-64.

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For research use only. Not intended for any clinical use.

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