Human NDUFS8 ELISA Kit (V2LY-0626-LY5649)

Name: Human NDUFS8 ELISA Kit
SKU: V2LY-0626-LY5649
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0034 ng/mL

Detection Range

0.007-1.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

23.7 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

NADH:UBIQUINONE OXIDOREDUCTASE CORE SUBUNIT S8

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:22499348).

Essential for the catalytic activity and assembly of complex I (PubMed:22499348).

Biological Process

Aerobic respiration Source: GO_Central
Mitochondrial ATP synthesis coupled proton transport Source: ComplexPortal
Mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
Mitochondrial respiratory chain complex I assembly Source: UniProtKB
Response to oxidative stress Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

Involvement in disease

Mitochondrial complex I deficiency, nuclear type 2 (MC1DN2):
A form of mitochondrial complex I deficiency, the most common biochemical signature of mitochondrial disorders, a group of highly heterogeneous conditions characterized by defective oxidative phosphorylation, which collectively affects 1 in 5-10000 live births. Clinical disorders have variable severity, ranging from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. MC1DN2 inheritance is autosomal recessive.

Alkhaldi, H. A., & Vik, S. B. (2023). Analysis of compound heterozygous and homozygous mutations found in peripheral subunits of human respiratory Complex I, NDUFS1, NDUFS2, NDUFS8 and NDUFV1, by modeling in the E. coli enzyme. Mitochondrion, 68, 87-104.

Andzelm, M. M., Balasubramaniam, S., Yang, E., Compton, A. G., Millington, K., Zhu, J., ... & Srivastava, S. (2022). Expansion of the clinical and neuroimaging spectrum associated with NDUFS8‐related disorder. JIMD reports, 63(5), 391-399.

Wang, S., Kang, Y., Wang, R., Deng, J., Yu, Y., Yu, J., & Wang, J. (2022). Emerging Roles of NDUFS8 Located in Mitochondrial Complex I in Different Diseases. Molecules, 27(24), 8754.

Flotyńska, J., Klause, D., Kulecki, M., Cieluch, A., Chomicka-Pawlak, R., Zozulińska-Ziółkiewicz, D., & Uruska, A. (2022). Higher NADH Dehydrogenase [Ubiquinone] Iron–Sulfur Protein 8 (NDUFS8) Serum Levels Correlate with Better Insulin Sensitivity in Type 1 Diabetes. Current Issues in Molecular Biology, 44(9), 3872-3883.

Lin, B. Y., Zheng, G. T., Teng, K. W., Chang, J. Y., Lee, C. C., Liao, P. C., & Kao, M. C. (2021). TAT-conjugated NDUFS8 can be transduced into mitochondria in a membrane-potential-independent manner and rescue complex I deficiency. International Journal of Molecular Sciences, 22(12), 6524.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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