Human PICALM ELISA Kit (V2LY-0626-LY3790)

Phosphatidylinositol Binding Clathrin Assembly Protein

Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PubMed:10436022, PubMed:16262731, PubMed:27574975).
Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PubMed:25898166).
In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PubMed:22118466, PubMed:21808019, PubMed:23741335).
In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PubMed:25241929).
Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF) (PubMed:25241929, PubMed:24067654).

Amyloid-beta clearance by transcytosisManual Assertion Based On ExperimentIGI:ARUK-UCL
AxonogenesisIEA:Ensembl
Clathrin coat assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Clathrin-coated pit assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Clathrin-dependent endocytosisManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Dendrite morphogenesisIEA:Ensembl
EndocytosisManual Assertion Based On ExperimentIDA:UniProtKB
Endosomal transportManual Assertion Based On ExperimentIMP:BHF-UCL
HemopoiesisIEA:Ensembl
Iron ion homeostasisManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Learning or memoryBy SimilarityISS:ARUK-UCL
Membrane bendingManual Assertion Based On ExperimentIMP:ARUK-UCL
Modulation of age-related behavioral declineBy SimilarityISS:ARUK-UCL
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic processISS:Alzheimers_University_of_Toronto
Negative regulation of protein localization to cell surfaceManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of protein localization to plasma membraneManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of receptor-mediated endocytosisManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of amyloid-beta formationManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic processISS:Alzheimers_University_of_Toronto
Positive regulation of GTPase activityManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of neuron deathManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:BHF-UCL
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Receptor internalizationManual Assertion Based On ExperimentIMP:BHF-UCL
Receptor-mediated endocytosisManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic processManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Regulation of clathrin-dependent endocytosisIEA:InterPro
Regulation of endocytosisManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of protein localizationManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of vesicle sizeManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process synaptic vesicle budding from presynaptic endocytic zone membraneManual Assertion Based On ExperimentIBA:GO_Central
Synaptic vesicle maturationISS:Alzheimers_University_of_Toronto
Vesicle budding from membraneManual Assertion Based On ExperimentIMP:ARUK-UCL
Vesicle cargo loadingManual Assertion Based On ExperimentIMP:Alzheimers_University_of_Toronto
Vesicle-mediated transportManual Assertion Based On ExperimentTAS:ProtInc

Cell membrane
Membrane, clathrin-coated pit
Golgi apparatus
Cytoplasmic vesicle, clathrin-coated vesicle
Nucleus
Colocalized with clathrin in the Golgi area (PubMed:10436022).
Interaction with PIMREG may target PICALM to the nucleus in some cells (PubMed:16491119).

A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.