Human PIM3 ELISA Kit (V2LY-0626-LY2873)

Name: Human PIM3 ELISA Kit
SKU: V2LY-0626-LY2873
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Basic Information

Sensitivity

0.013 ng/mL

Detection Range

0.02-4.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

35.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Pim-3 Proto-Oncogene, Serine/Threonine Kinase

Function

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

Biological Process

Apoptotic processIEA:UniProtKB-KW
Cell cycleIEA:UniProtKB-KW
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of insulin secretion involved in cellular response to glucose stimulusISS:UniProtKB
Protein autophosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm

PTM

Ubiquitinated, leading to proteasomal degradation.
Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PIM3, Active protein, GST Tag (CAT#: V2LY-0526-LY6188)

Mouse Recombinant PIM3, Active protein, GST Tag (CAT#: V2LY-0526-LY8782)

Related Products

Mouse Anti-PIM3 Recombinant Antibody (4A9) (CAT#: CBMAB-P1855-YC)

Rabbit Anti-PIM3 Recombinant Antibody (D17C9) (CAT#: CBMAB-P1856-YC)

Mouse Recombinant PIM3, Active protein, GST Tag (CAT#: V2LY-0526-LY8782)

Human Recombinant PIM3, Active protein, GST Tag (CAT#: V2LY-0526-LY6188)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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