Human PLIN5 ELISA Kit (V2LY-0626-LY5472)

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Tested Data
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Basic Information

Sensitivity
0.012 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
50.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
PERILIPIN 5
Function
Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE (By similarity).
Biological Process
Lipid droplet organizationISS:UniProtKB
Lipid storageManual Assertion Based On ExperimentIBA:GO_Central
Mitochondrion localizationIEA:Ensembl
Negative regulation of fatty acid beta-oxidationISS:UniProtKB
Negative regulation of lipase activityISS:UniProtKB
Negative regulation of peroxisome proliferator activated receptor signaling pathwayISS:UniProtKB
Negative regulation of reactive oxygen species metabolic processISS:UniProtKB
Negative regulation of triglyceride catabolic processISS:UniProtKB
Positive regulation of fatty acid beta-oxidationISS:UniProtKB
Positive regulation of lipase activityISS:UniProtKB
Positive regulation of lipid storageISS:UniProtKB
Positive regulation of sequestering of triglycerideISS:UniProtKB
Positive regulation of triglyceride biosynthetic processISS:UniProtKB
Cellular Location
Lipid droplet
Cytoplasm
Mitochondrion
Lipid droplet surface-associated. Exchanges between lipid droplets and the cytoplasm.
PTM
Phosphorylated by PKA. Phosphorylated on serine in skeletal muscle at rest or upon lipolytic stimulation (By similarity).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PLIN5 Recombinant Antibody (CBYJL-2055) (CAT#: CBMAB-L2183-YJ)

Isotype control

For research use only. Not intended for any clinical use.

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