Human ARID1A ELISA Kit (2) (V2LY-0626-LY5496)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0079 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
242.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
AT-Rich Interaction Domain 1A
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Biological Process
Androgen receptor signaling pathway Source: UniProtKB
ATP-dependent chromatin remodeling Source: GO_Central
Chromatin-mediated maintenance of transcription Source: UniProtKB
Chromatin remodeling Source: BHF-UCL
Glucocorticoid receptor signaling pathway Source: UniProtKB
Intracellular estrogen receptor signaling pathway Source: UniProtKB
Nervous system development Source: UniProtKB-KW
Nucleosome disassembly Source: BHF-UCL
Nucleosome mobilization Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
Coffin-Siris syndrome 2 (CSS2): A form of Coffin-Siris syndrome, a congenital multiple malformation syndrome with broad phenotypic and genetic variability. Cardinal features are intellectual disability, coarse facial features, hypertrichosis, and hypoplastic or absent fifth digit nails or phalanges. Additional features include malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems. Sucking/feeding difficulties, poor growth, ophthalmologic abnormalities, hearing impairment, and spinal anomalies are common findings. Both autosomal dominant and autosomal recessive inheritance patterns have been reported.

Lee, C. G., & Ki, C. S. (2021). A Novel De Novo Heterozygous ARID1A Missense Variant Cluster in cis c.[5954C> G; 6314C> T; 6334C> T; 6843G> C] causes a Coffin–Siris Syndrome. Annals of Laboratory Medicine, 41(3), 350.

Qadir, J., Majid, S., Khan, M. S., Rashid, F., Wani, M. D., Din, I., & Bashir, H. (2020). AT-rich interaction domain 1A gene variations: genetic associations and susceptibility to gastric cancer risk. Pathology & Oncology Research, 26(4), 2237-2246.

Okamura, R., Kato, S., Lee, S., Jimenez, R. E., Sicklick, J. K., & Kurzrock, R. (2020). ARID1A alterations function as a biomarker for longer progression-free survival after anti-PD-1/PD-L1 immunotherapy. Journal for immunotherapy of cancer, 8(1).

Ferri-Borgogno, S., Barui, S., McGee, A. M., Griffiths, T., Singh, P. K., Piett, C. G., ... & Gupta, S. (2020). Paradoxical role of AT-rich interactive domain 1A in restraining pancreatic carcinogenesis. Cancers, 12(9), 2695.

Hu, G., Tu, W., Yang, L., Peng, G., & Yang, L. (2020). ARID1A deficiency and immune checkpoint blockade therapy: From mechanisms to clinical application. Cancer letters, 473, 148-155.

Raffone, A., Travaglino, A., Saccone, G., Cieri, M., Mascolo, M., Mollo, A., ... & Zullo, F. (2019). Diagnostic and prognostic value of ARID1A in endometrial hyperplasia: a novel marker of occult cancer. Apmis, 127(9), 597-606.

Zhou, H., Tan, S., Li, H., & Lin, X. (2019). Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma. Molecular medicine reports, 19(3), 2125-2136.

Bui, C. B., Le, H. K., Vu, D. M., Truong, K. D. D., Nguyen, N. M., Ho, M. A. N., & Truong, D. Q. (2019). ARID1A‐SIN3A drives retinoic acid‐induced neuroblastoma differentiation by transcriptional repression of TERT. Molecular carcinogenesis, 58(11), 1998-2007.

Zhang, L., Wang, C., Yu, S., Jia, C., Yan, J., Lu, Z., & Chen, J. (2018). Loss of ARID1A expression correlates with tumor differentiation and tumor progression stage in pancreatic ductal adenocarcinoma. Technology in cancer research & treatment, 17, 1533034618754475.

Hu, C., Li, W., Tian, F., Jiang, K., Liu, X., Cen, J., ... & Hui, L. (2018). Arid1a regulates response to anti-angiogenic therapy in advanced hepatocellular carcinoma. Journal of hepatology, 68(3), 465-475.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human ARID1A ELISA Kit (CAT#: V2LY-0626-LY4007)

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Human ARID1A ELISA Kit (CAT#: V2LY-0626-LY4007)

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For research use only. Not intended for any clinical use.

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