Human RACGAP1 ELISA Kit (V2LY-0626-LY4283)
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Basic Information
- Pre-coated ELISA Plate: 12 wells * 8 detachable strips
- Standard solution: 0.5ml x1
- Standard diluent: 3ml x1
- Streptavidin-HRP: 6ml x1
- Stop solution: 6ml x1
- Substrate solution A: 6ml x1
- Substrate solution B: 6ml x1
- Wash buffer concentrate (25x): 20ml x1
- Biotinylated antibody: 1ml x1
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Biological Process mitotic cytokinesisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process mitotic spindle midzone assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process neuroblast proliferationISS:UniProtKB
Biological Process positive regulation of cytokinesisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of attachment of spindle microtubules to kinetochoreManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of embryonic developmentISS:UniProtKB
Biological Process regulation of small GTPase mediated signal transductionTAS:Reactome
Biological Process Rho protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process spermatogenesisManual Assertion Based On ExperimentIEP:UniProtKB
Biological Process sulfate transportManual Assertion Based On ExperimentIDA:UniProtKB
Cytoplasm
Cytoplasm, cytoskeleton, spindle
Cytoplasmic vesicle, secretory vesicle, acrosome
Cleavage furrow
Midbody, Midbody ring
Cell membrane
Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity).
During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody ring, also called Flemming body. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA (PubMed:12689593).
Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation (PubMed:19468302, PubMed:19468300).
Phosphorylation on Ser-164 plays a major role in mediating interaction with ECT2 (PubMed:25068414).
Phosphorylation on Ser-157 does not appear to contribute to binding to ECT2 (PubMed:25068414).
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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