Human Recombinant AKR1C2 protein, His Tag (V2LY-0526-LY1970)

Name: Human Recombinant AKR1C2 protein, His Tag
SKU: V2LY-0526-LY1970
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant AKR1C2 protein, His Tag consist of Amino Acid: 1-323 and predicts a molecular mass of 38.6 kDa.

Molecule Mass

38.6 kDa

Sequence

Amino Acid: 1-323

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Glycerol, Arg, PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Aldo-Keto Reductase Family 1 Member C2

Function

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:15929998, PubMed:17034817, PubMed:17442338, PubMed:8573067). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT (PubMed:10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699).

Biological Process

Cellular response to jasmonic acid stimulus Source: UniProtKB
Cellular response to prostaglandin D stimulus Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
Digestion Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Progesterone metabolic process Source: UniProtKB
Prostaglandin metabolic process Source: UniProtKB
Steroid metabolic process Source: UniProtKB

Cellular Location

Cytosol

Involvement in disease

46,XY sex reversal 8 (SRXY8): A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.

Sun, T., Gao, L., Sun, X., Wang, X., Guo, R., & Yu, Y. (2021). Aldo-keto reductase 1C inhibitors could reverse resistance of HepG2 Cis-platin-resistant cells, but not only by inhibiting AKR1Cs, based on transcriptomic and NADH metabolic analysis. bioRxiv.

Franko, A., Berti, L., Hennenlotter, J., Rausch, S., Scharpf, M. O., de Angelis, M. H., ... & Heni, M. (2020). Transcript Levels of Aldo-Keto Reductase Family 1 Subfamily C (AKR1C) Are Increased in Prostate Tissue of Patients with Type 2 Diabetes. Journal of personalized medicine, 10(3), 124.

Jin, Y. X., Zhou, X. F., Chen, Y. Y., Jin, W. X., Wang, Y. H., Ye, D. R., ... & Chen, E. D. (2019). Up-Regulated AKR1C2 is correlated with favorable prognosis in thyroid carcinoma. Journal of Cancer, 10(15), 3543.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

AKR1C2 Matched Antibody Pair (052) (CAT#: APMAB-052LY)

AKR1C2 Matched Antibody Pair (053) (CAT#: APMAB-053LY)

Antibody Pairs

AKR1C2 Matched Antibody Pair (052) (CAT#: APMAB-052LY)

AKR1C2 Matched Antibody Pair (053) (CAT#: APMAB-053LY)

Related Products

AKR1C2 Matched Antibody Pair (052) (CAT#: APMAB-052LY)

Mouse Anti-AKR1C2 Recombinant Antibody (V2-49560) (CAT#: CBMAB-0009-YY)

AKR1C2 Matched Antibody Pair (053) (CAT#: APMAB-053LY)

Mouse Anti-AKR1C2 Recombinant Antibody (V2-180477) (CAT#: CBMAB-A1990-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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