Human Recombinant CD300LG protein, hFc Tag (V2LY-0526-LY3242)

Name: Human Recombinant CD300LG protein, hFc Tag
SKU: V2LY-0526-LY3242
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CD300LG protein, hFc Tag consist of Amino Acid: 1-247 and predicts a molecular mass of 51.7 kDa.

Molecule Mass

51.7 kDa

Sequence

Amino Acid: 1-247

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD300 Molecule Like Family Member G

Function

Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner. Binds lymphocyte (By similarity).

Biological Process

Immune system process Source: UniProtKB-KW
Regulation of immune response Source: Reactome

Cellular Location

Multivesicular body membrane; Apical cell membrane; Basolateral cell membrane. Transcytoses across the cytoplasm.

Topology

Extracellular: 19-247
Helical: 248-268
Cytoplasmic: 269-332

PTM

O-glycosylated with sialylated oligosaccharides.

Mamoor, S. (2021). Differential expression of CD300 molecule-like family member g in cancers of the breast.

Mamoor, S. (2020). CD300LG (Nepmucin) is differentially expressed in brain metastatic breast cancer.

Wang, Q., Liu, Y., Chen, Y., Wang, K., Xie, W., Wei, D., & Hu, L. (2017). CD300LG improves the cytotoxic activity of CIK. Central-European journal of immunology, 42(2), 117.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD300LG protein (CAT#: V2LY-0526-LY3241)

Human Recombinant CD300LG protein, His Tag (CAT#: V2LY-0526-LY3243)

Rat Recombinant CD300LG protein, His Tag (CAT#: V2LY-0526-LY9296)

Related Products

Rat Recombinant CD300LG protein, His Tag (CAT#: V2LY-0526-LY9296)

Rabbit Anti-CD300LG Recombinant Antibody (CBFYC-1215) (CAT#: CBMAB-C1273-FY)

Human Recombinant CD300LG protein, His Tag (CAT#: V2LY-0526-LY3243)

Human Recombinant CD300LG protein (CAT#: V2LY-0526-LY3241)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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