Human Recombinant CD34 protein, hFc Tag (V2LY-0526-LY2813)

Name: Human Recombinant CD34 protein, hFc Tag
SKU: V2LY-0526-LY2813
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CD34 protein, hFc Tag consist of Amino Acid: 1-290 and predicts a molecular mass of 54 kDa.

Molecule Mass

54 kDa

Verified

HPLC

Sequence

Amino Acid: 1-290

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD34 Molecule

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Biological Process

Cell-cell adhesion Source: HGNC-UCL
Cell-matrix adhesion Source: GO_Central
Cell motility Source: UniProtKB
Endothelial cell proliferation Source: UniProtKB
Endothelium development Source: UniProtKB
Extracellular exosome assembly Source: UniProtKB
Glomerular endothelium development Source: UniProtKB
Glomerular filtration Source: UniProtKB
Hematopoietic stem cell proliferation Source: UniProtKB
Hemopoiesis Source: UniProtKB
Leukocyte migration Source: HGNC-UCL
Mesangial cell-matrix adhesion Source: UniProtKB
Metanephric glomerular mesangial cell differentiation Source: UniProtKB
Negative regulation of blood coagulation Source: UniProtKB
Negative regulation of cellular response to heat Source: UniProtKB
Negative regulation of cellular response to hypoxia Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative regulation of interleukin-2 production Source: UniProtKB
Negative regulation of neuron death Source: UniProtKB
Negative regulation of nitric oxide biosynthetic process Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: UniProtKB
Paracrine signaling Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of glial cell-derived neurotrophic factor production Source: UniProtKB
Positive regulation of granulocyte colony-stimulating factor production Source: UniProtKB
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of odontogenesis Source: UniProtKB
Positive regulation of transforming growth factor beta production Source: UniProtKB
Positive regulation of vasculogenesis Source: UniProtKB
Regulation of blood pressure Source: UniProtKB
Regulation of immune response Source: Reactome
Signal transduction Source: UniProtKB
Stem cell proliferation Source: UniProtKB
Tissue homeostasis Source: UniProtKB
Transdifferentiation Source: UniProtKB
Vascular wound healing Source: UniProtKB

Cellular Location

Membrane

Topology

Extracellular: 32-290
Helical: 291-311
Cytoplasmic: 312-385

PTM

Highly glycosylated.
Phosphorylated on serine residues by PKC.

Abs, D., Landman, S., Osio, A., Lepesant, P., Schneider, P., Obadia, D., ... & Battistella, M. (2021). Spindle cell tumor with CD34 and S100 co‐expression and distinctive stromal and perivascular hyalinization showing EML4‐ALK fusion. Journal of Cutaneous Pathology, 48(7), 896-901.

Michal, M., Ptáková, N., Martínek, P., Gatalica, Z., Kazakov, D. V., Michalová, K., ... & Michal, M. (2019). S100 and CD34 positive spindle cell tumor with prominent perivascular hyalinization and a novel NCOA4‐RET fusion. Genes, Chromosomes and Cancer, 58(9), 680-685.

Michaelsen, S. R., Urup, T., Olsen, L. R., Broholm, H., Lassen, U., & Poulsen, H. S. (2018). Molecular profiling of short-term and long-term surviving patients identifies CD34 mRNA level as prognostic for glioblastoma survival. Journal of Neuro-oncology, 137(3), 533-542.

Bellagamba, B. C., Grudzinski, P. B., Ely, P. B., Nader, P. D. J. H., Nardi, N. B., & da Silva Meirelles, L. (2018). Induction of expression of CD271 and CD34 in mesenchymal stromal cells cultured as spheroids. Stem cells international, 2018.

Sumide, K., Matsuoka, Y., Kawamura, H., Nakatsuka, R., Fujioka, T., Asano, H., ... & Sonoda, Y. (2018). A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. Nature communications, 9(1), 1-17.

Suurmeijer, A. J., Dickson, B. C., Swanson, D., Zhang, L., Sung, Y. S., Cotzia, P., ... & Antonescu, C. R. (2018). A novel group of spindle cell tumors defined by S100 and CD34 co‐expression shows recurrent fusions involving RAF1, BRAF, and NTRK1/2 genes. Genes, Chromosomes and Cancer, 57(12), 611-621.

Fan, J., Li, H., Nie, X., Yin, Z., Zhao, Y., Zhang, X., ... & Wang, D. W. (2018). MiR-665 aggravates heart failure via suppressing CD34-mediated coronary microvessel angiogenesis. Aging (Albany NY), 10(9), 2459.

Abe, T., Matsuoka, Y., Nagao, Y., Sonoda, Y., & Hanazono, Y. (2017). CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep. International journal of hematology, 106(5), 631-637.

Huse, J. T., Snuderl, M., Jones, D. T., Brathwaite, C. D., Altman, N., Lavi, E., ... & Rosenblum, M. K. (2017). Polymorphous low-grade neuroepithelial tumor of the young (PLNTY): an epileptogenic neoplasm with oligodendroglioma-like components, aberrant CD34 expression, and genetic alterations involving the MAP kinase pathway. Acta neuropathologica, 133(3), 417-429.

Foroozan, M., Roudi, R., Abolhasani, M., Gheytanchi, E., & Mehrazma, M. (2017). Clinical significance of endothelial cell marker CD34 and mast cell marker CD117 in prostate adenocarcinoma. Pathology-Research and Practice, 213(6), 612-618.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD34 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2811)

Human Recombinant CD34 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY2812)

Human Recombinant CD34 protein, His Tag (CAT#: V2LY-0526-LY2814)

Mouse Recombinant CD34 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8001)

Mouse Recombinant CD34 protein, His Tag (CAT#: V2LY-0526-LY8002)

Rat Recombinant CD34 protein, hFc Tag (CAT#: V2LY-0526-LY9236)

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Isotype control

For research use only. Not intended for any clinical use.

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