Human Recombinant CLK1, Active protein, GST Tag (V2LY-0526-LY3228)

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Basic Information

Expressed Host
Baculovirus-Insect Cells
Protein Species
Human
Tag
GST Tag
Protein Construction
This product is Human Recombinant CLK1, Active protein, GST Tag consist of Amino Acid: 129-end and predicts a molecular mass of 66 kDa.
Molecule Mass
66 kDa
Sequence
Amino Acid: 129-end
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
Batch dependent.
Endotoxin
Please contact us for more information.
Format
Liquid
Buffer
Tirs, NaCl, Glycerol
Preservative
None
Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
More Infomation

Target

Full Name
CDC Like Kinase 1
Function
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
Biological Process
Peptidyl-serine phosphorylation Source: Ensembl
Peptidyl-threonine phosphorylation Source: Ensembl
Protein autophosphorylation Source: Ensembl
Regulation of RNA splicing Source: UniProtKB
Cellular Location
Nucleus
PTM
Autophosphorylates on all three types of residues.

Dekel, N., Eisenberg-Domovich, Y., Karlas, A., Meyer, T. F., Bracher, F., Lebendiker, M., ... & Livnah, O. (2020). Expression, purification and crystallization of CLK1 kinase–A potential target for antiviral therapy. Protein Expression and Purification, 176, 105742.

Babu, N., Pinto, S. M., Biswas, M., Subbannayya, T., Rajappa, M., Mohan, S. V., ... & Chatterjee, A. (2020). Phosphoproteomic analysis identifies CLK1 as a novel therapeutic target in gastric cancer. Gastric Cancer, 23(5), 796-810.

Artarini, A., Meyer, M., Shin, Y. J., Huber, K., Hilz, N., Bracher, F., ... & Karlas, A. (2019). Regulation of influenza A virus mRNA splicing by CLK1. Antiviral research, 168, 187-196.

Sun, L., Cheong, J. E., Zaffagni, M., Zhou, K., & Zetter, B. (2018). Discovery of novel water-soluble derivatives of mebendazole as selective CLK1/4 kinase inhibitors and their anticancer cancer activity.

Uzor, S., Zorzou, P., Bowler, E., Porazinski, S., Wilson, I., & Ladomery, M. (2018). Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention. Gene, 670, 46-54.

Sako, Y., Ninomiya, K., Okuno, Y., Toyomoto, M., Nishida, A., Koike, Y., ... & Hagiwara, M. (2017). Development of an orally available inhibitor of CLK1 for skipping a mutated dystrophin exon in Duchenne muscular dystrophy. Scientific reports, 7(1), 1-9.

Sun, Q. Z., Lin, G. F., Li, L. L., Jin, X. T., Huang, L. Y., Zhang, G., ... & Yang, S. Y. (2017). Discovery of potent and selective inhibitors of cdc2-like kinase 1 (clk1) as a new class of autophagy inducers. Journal of medicinal chemistry, 60(14), 6337-6352.

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For research use only. Not intended for any clinical use.

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