Human Recombinant CLK2, Active protein, GST Tag (V2LY-0526-LY3229)

Name: Human Recombinant CLK2, Active protein, GST Tag
SKU: V2LY-0526-LY3229
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant CLK2, Active protein, GST Tag consist of Amino Acid: 137-end and predicts a molecular mass of 68 kDa.

Molecule Mass

68 kDa

Sequence

Amino Acid: 137-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

CDC Like Kinase 2

Function

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Phosphorylates PAGE4 at several serine and threonine residues and this phosphorylation attenuates the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed:28289210).

Biological Process

Negative regulation of gluconeogenesis Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of RNA splicing Source: UniProtKB
Response to ionizing radiation Source: UniProtKB

Cellular Location

Nucleus
Isoform 1: Nucleus; Nucleus speckle. Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.
Isoform 2: Nucleus speckle. Co-localizes with serine- and arginine-rich (SR) proteins in the nuclear speckles.

PTM

Autophosphorylates on all three types of residues. Phosphorylation on Ser-34 and Thr-127 by AKT1 is induced by ionizing radiation or insulin. Phosphorylation plays a critical role in cell proliferation following low dose radiation and prevents cell death following high dose radiation. Phosphorylation at Thr-344 by PKB/AKT2 induces its kinase activity which is required for its stability. The phosphorylation status at Ser-142 influences its subnuclear localization; inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.

Qin, Z., Qin, L., Feng, X., Li, Z., & Bian, J. (2021). Development of Cdc2-like Kinase 2 Inhibitors: Achievements and Future Directions. Journal of Medicinal Chemistry, 64(18), 13191-13211.

Liu, B., Kong, X., Wang, R., & Xin, C. (2021). CLK2 promotes occurrence and development of non-small cell lung cancer. Journal of BU ON.: Official Journal of the Balkan Union of Oncology, 26(1), 58-64.

Licciardello, M. P., & Workman, P. (2021). A New Chemical Probe Challenges the Broad Cancer Essentiality of CK2. Trends in Pharmacological Sciences.

Yazici, Y., McAlindon, T. E., Gibofsky, A., Lane, N. E., Clauw, D., Jones, M., ... & Hochberg, M. C. (2020). Lorecivivint, a Novel Intraarticular CDC‐like Kinase 2 and Dual‐Specificity Tyrosine Phosphorylation‐Regulated Kinase 1A Inhibitor and Wnt Pathway Modulator for the Treatment of Knee Osteoarthritis: A Phase II Randomized Trial. Arthritis & Rheumatology, 72(10), 1694-1706.

Wu, Z. X., Yang, Y., Wang, G., Wang, J. Q., Teng, Q. X., Sun, L., ... & Zou, C. (2020). Dual TTK/CLK2 inhibitor, CC‐671, selectively antagonizes ABCG2‐mediated multidrug resistance in lung cancer cells. Cancer Science, 111(8), 2872.

Deshmukh, V., O'Green, A. L., Bossard, C., Seo, T., Lamangan, L., Ibanez, M., ... & Yazici, Y. (2019). Modulation of the Wnt pathway through inhibition of CLK2 and DYRK1A by lorecivivint as a novel, potentially disease-modifying approach for knee osteoarthritis treatment. Osteoarthritis and cartilage, 27(9), 1347-1360.

Hatting, M., Rines, A. K., Luo, C., Tabata, M., Sharabi, K., Hall, J. A., ... & Puigserver, P. (2017). Adipose tissue CLK2 promotes energy expenditure during high-fat diet intermittent fasting. Cell metabolism, 25(2), 428-437.

Quaresma, P. G. F., Weissmann, L., Zanotto, T. M., Santos, A. C., De Matos, A. H. B., Furigo, I. C., ... & Prada, P. O. (2017). Cdc2-like kinase 2 in the hypothalamus is necessary to maintain energy homeostasis. International Journal of Obesity, 41(2), 268-278.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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