Human Recombinant COL5A2 protein, Fc Tag (V2LY-0526-LY3314)

Name: Human Recombinant COL5A2 protein, Fc Tag
SKU: V2LY-0526-LY3314
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

Fc Tag

Protein Construction

This product is Human Recombinant COL5A2 protein, Fc Tag consist of Amino Acid: 1-1229 and predicts a molecular mass of 138.6 kDa.

Molecule Mass

138.6 kDa

Sequence

Amino Acid: 1-1229

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Collagen Type V Alpha 2 Chain

Function

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices (By similarity).

Biological Process

Cellular response to amino acid stimulus Source: Ensembl
Collagen fibril organization Source: UniProtKB
Extracellular matrix organization Source: GO_Central
Eye morphogenesis Source: UniProtKB
Negative regulation of endodermal cell differentiation Source: UniProtKB
Ossification Source: Ensembl
Skeletal system development Source: Ensembl
Skin development Source: UniProtKB

Cellular Location

Extracellular matrix

Involvement in disease

Ehlers-Danlos syndrome, classic type, 2 (EDSCL2):
A form of Ehlers-Danlos syndrome, a group of connective tissue disorders characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. The main features of classic Ehlers-Danlos syndrome are joint hypermobility and dislocation, and fragile, bruisable skin. EDSCL2 inheritance is autosomal dominant.

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Probably 3-hydroxylated on Pro-919 and Pro-1156 by LEPREL1.

Mamoor, S. (2021). COL5A2 is differentially expressed in lymph node metastasis in human breast cancer.

Ilieș, R. F., Aioanei, C. S., Cătană, A., Halmagyi, S. R., Lukacs, I., Tokes, R. E., ... & Pop, I. V. (2021). Involvement of COL5A2 and TGF‑β1 in pathological scarring. Experimental and Therapeutic Medicine, 22(4), 1-4.

Chen, H. C., Tseng, Y. K., Shu, C. W., Weng, T. J., Liou, H. H., Yen, L. M., ... & Liu, P. F. (2019). Differential clinical significance of COL 5A1 and COL 5A2 in tongue squamous cell carcinoma. Journal of Oral Pathology & Medicine, 48(6), 468-476.

Dong, Y., Yang, L., Luo, W., Zhu, T., Yan, W., Kong, J., ... & Zhao, Q. (2019). Mannose receptor C type 2 mediates 1, 25 (OH) 2D3/vitamin D receptor-regulated collagen metabolism through collagen type 5, alpha 2 chain and matrix metalloproteinase 13 in murine MC3T3-E1 cells. Molecular and cellular endocrinology, 483, 74-86.

Meng, X. Y., Shi, M. J., Zeng, Z. H., Chen, C., Liu, T. Z., Wu, Q. J., ... & Li, S. (2019). The role of COL5A2 in patients with muscle-invasive bladder cancer: a bioinformatics analysis of public datasets involving 787 subjects and 29 cell lines. Frontiers in Oncology, 8, 659.

Zeng, X. T., Liu, X. P., Liu, T. Z., & Wang, X. H. (2018). The clinical significance of COL5A2 in patients with bladder cancer: A retrospective analysis of bladder cancer gene expression data. Medicine, 97(10).

Yang, F., Luo, P., Ding, H., Zhang, C., & Zhu, Z. (2018). Collagen type V a2 (COL5A2) is decreased in steroid-induced necrosis of the femoral head. American Journal of Translational Research, 10(8), 2469.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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