Human Recombinant CSF1 protein, hFc Tag (V2LY-0526-LY5484)

Name: Human Recombinant CSF1 protein, hFc Tag
SKU: V2LY-0526-LY5484
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CSF1 protein, hFc Tag consist of Amino Acid: 1-255 and predicts a molecular mass of 51.9 kDa.

Molecule Mass

51.9 kDa

Sequence

Amino Acid: 1-255

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Colony Stimulating Factor 1

Function

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

Biological Process

Branching involved in mammary gland duct morphogenesis Source: Ensembl
Cellular protein metabolic process Source: Reactome
Cytokine-mediated signaling pathway Source: Reactome
Developmental process involved in reproduction Source: BHF-UCL
Homeostasis of number of cells within a tissue Source: Ensembl
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Macrophage colony-stimulating factor signaling pathway Source: Ensembl
Macrophage differentiation Source: UniProtKB
Mammary duct terminal end bud growth Source: Ensembl
Mammary gland fat development Source: Ensembl
Microglial cell proliferation Source: Ensembl
Monocyte activation Source: BHF-UCL
Negative regulation of neuron death Source: ARUK-UCL
Osteoclast differentiation Source: BHF-UCL
Osteoclast proliferation Source: Ensembl
Positive regulation of cell-matrix adhesion Source: BHF-UCL
Positive regulation of cell migration Source: BHF-UCL
Positive regulation of cell population proliferation Source: MGI
Positive regulation of cellular protein metabolic process Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of macrophage chemotaxis Source: BHF-UCL
Positive regulation of macrophage colony-stimulating factor signaling pathway Source: BHF-UCL
Positive regulation of macrophage derived foam cell differentiation Source: BHF-UCL
Positive regulation of macrophage differentiation Source: BHF-UCL
Positive regulation of microglial cell migration Source: BHF-UCL
Positive regulation of monocyte differentiation Source: BHF-UCL
Positive regulation of mononuclear cell proliferation Source: BHF-UCL
Positive regulation of multicellular organism growth Source: Ensembl
Positive regulation of odontogenesis of dentin-containing tooth Source: Ensembl
Positive regulation of osteoclast differentiation Source: BHF-UCL
Positive regulation of protein kinase activity Source: BHF-UCL
Positive regulation of Ras protein signal transduction Source: Ensembl
Post-translational protein modification Source: Reactome
Regulation of macrophage derived foam cell differentiation Source: BHF-UCL
Regulation of ossification Source: Ensembl
Response to ischemia Source: ARUK-UCL
Transmembrane receptor protein tyrosine kinase signaling pathway Source: BHF-UCL

Cellular Location

Cell membrane
Processed macrophage colony-stimulating factor 1: Extracellular space

Involvement in disease

Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers.
Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection.

Topology

Lumenal: 33-496
Helical: 497-517
Cytoplasmic: 518-554

PTM

N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.

Yu, X., Basbaum, A., & Guan, Z. (2021). Contribution of colony-stimulating factor 1 to neuropathic pain. Pain reports, 6(1).

Kaur, S., Sehgal, A., Wu, A. C., Millard, S. M., Batoon, L., Sandrock, C. J., ... & Pettit, A. R. (2021). Stable colony-stimulating factor 1 fusion protein treatment increases hematopoietic stem cell pool and enhances their mobilisation in mice. Journal of Hematology & Oncology, 14(1), 1-19.

Pognan, F., Couttet, P., Demin, I., Jaitner, B., Pang, Y., Roubenoff, R., ... & Schramm, U. (2019). Colony-stimulating factor-1 antibody lacnotuzumab in a phase 1 healthy volunteer study and mechanistic investigation of safety outcomes. Journal of Pharmacology and Experimental Therapeutics, 369(3), 428-442.

Zhu, Y., Yang, J., Xu, D., Gao, X. M., Zhang, Z., Hsu, J. L., ... & Qin, L. X. (2019). Disruption of tumour-associated macrophage trafficking by the osteopontin-induced colony-stimulating factor-1 signalling sensitises hepatocellular carcinoma to anti-PD-L1 blockade. Gut, 68(9), 1653-1666.

Okugawa, Y., Toiyama, Y., Ichikawa, T., Kawamura, M., Yasuda, H., Fujikawa, H., ... & Kusunoki, M. (2018). Colony-stimulating factor-1 and colony-stimulating factor-1 receptor co-expression is associated with disease progression in gastric cancer. International journal of oncology, 53(2), 737-749.

Kumari, A., Silakari, O., & Singh, R. K. (2018). Recent advances in colony stimulating factor-1 receptor/c-FMS as an emerging target for various therapeutic implications. Biomedicine & Pharmacotherapy, 103, 662-679.

Wohleb, E. S., Terwilliger, R., Duman, C. H., & Duman, R. S. (2018). Stress-induced neuronal colony stimulating factor 1 provokes microglia-mediated neuronal remodeling and depressive-like behavior. Biological psychiatry, 83(1), 38-49.

Cannarile, M. A., Weisser, M., Jacob, W., Jegg, A. M., Ries, C. H., & Rüttinger, D. (2017). Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy. Journal for immunotherapy of cancer, 5(1), 1-13.

Papadopoulos, K. P., Gluck, L., Martin, L. P., Olszanski, A. J., Tolcher, A. W., Ngarmchamnanrith, G., ... & Stephenson, J. (2017). First-in-human study of AMG 820, a monoclonal anti-colony-stimulating factor 1 receptor antibody, in patients with advanced solid tumors. Clinical Cancer Research, 23(19), 5703-5710.

Walker, D. G., Tang, T. M., & Lue, L. F. (2017). Studies on colony stimulating factor receptor-1 and ligands colony stimulating factor-1 and interleukin-34 in Alzheimer's disease brains and human microglia. Frontiers in aging neuroscience, 9, 244.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus, Rhesus Recombinant CSF1 protein, hFc Tag (CAT#: V2LY-0526-LY1728)

Cynomolgus, Rhesus Recombinant CSF1 protein, His Tag (CAT#: V2LY-0526-LY1729)

Human Recombinant CSF1 protein (CAT#: V2LY-0526-LY5483)

Human Recombinant CSF1 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5485)

Human Recombinant CSF1 protein, His Tag (CAT#: V2LY-0526-LY5486)

Human Recombinant CSF1 protein, His Tag-1 (CAT#: V2LY-0526-LY5487)

Human Recombinant CSF1 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY5488)

Human Recombinant CSF1 protein, Biotin Conjugated (CAT#: V2LY-0526-LY5489)

Human Recombinant CSF1 protein-1 (CAT#: V2LY-0526-LY5490)

Mouse Recombinant CSF1 proteinECD (CAT#: V2LY-0526-LY8651)

Antibody Pairs

CSF1 Matched Antibody Pair (304) (CAT#: APMAB-304LY)

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CSF1 Matched Antibody Pair (304) (CAT#: APMAB-304LY)

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Mouse Anti-CSF1 Recombinant Antibody (2D10) (CAT#: CBMAB-C4849-LY)

Mouse Anti-CSF1 Recombinant Antibody (CBFYM-0100) (CAT#: V2LY-1206-LY1382)

Rabbit Anti-CSF1 Recombinant Antibody (CBFYM-1036) (CAT#: V2LY-1206-LY1394)

Mouse Anti-CSF1 Recombinant Antibody (CBFYM-0469) (CAT#: V2LY-1206-LY1393)

Human Recombinant CSF1 protein, Biotin Conjugated (CAT#: V2LY-0526-LY5489)

Rabbit Anti-CSF1 Recombinant Antibody (CBFYM-0184) (CAT#: V2LY-1206-LY1385)

Human Anti-CSF1 Recombinant Antibody (CBFYM-0334) (CAT#: V2LY-1206-LY1391)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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