Human Recombinant DCUN1D1 protein, His Tag (V2LY-0526-LY3514)

Name: Human Recombinant DCUN1D1 protein, His Tag
SKU: V2LY-0526-LY3514
Rating: 5 (1 reviews)

See all DCUN1D1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant DCUN1D1 protein, His Tag consist of Amino Acid: 1-259 and predicts a molecular mass of 32 kDa.

Molecule Mass

32 kDa

Sequence

Amino Acid: 1-259

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)

Function

Part of an E3 ubiquitin ligase complex for neddylation (PubMed:18826954).

Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes (PubMed:26906416, PubMed:23201271, PubMed:19617556, PubMed:23401859).

Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed:25349211, PubMed:28581483).

Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).

Biological Process

Positive regulation of protein neddylation Source: UniProtKB
Positive regulation of ubiquitin-protein transferase activity Source: GO_Central
Post-translational protein modification Source: Reactome
Protein neddylation Source: GO_Central
Regulation of protein neddylation Source: UniProtKB
Regulation of protein ubiquitination Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. The ubiquitinated form is localized in the cytoplasm.

PTM

Mono- and poly-ubiquitinated by ARIH2 and ARIH1. Monoubiquitination by ARIH2 is mediated by an interaction between autoubiquitinated ARIH2 and the UBA-like domain. The monoubiquitinated form preferentially interacts with non-neddylated cullins and modulates cullin RING ligase (CRL) complex composition and activity.

Huang, S. T., Liu, Z. Z., Jiang, F. N., He, H. C., & Zhong, W. D. (2021). DCUN1D1 promotes tumor progress in prostate cancer and its effect on DU145 in vitro. Journal of the Pakistan Medical Association, 71(2), 473-478.

Li, J., Yu, T., Yan, M., Zhang, X., Liao, L., Zhu, M., ... & Yao, M. (2019). DCUN1D1 facilitates tumor metastasis by activating FAK signaling and up-regulates PD-L1 in non-small-cell lung cancer. Experimental Cell Research, 374(2), 304-314.

Huang, G., Kaufman, A. J., Ryan, R. J., Romin, Y., Huryn, L., Bains, S., ... & Singh, B. (2019). Mouse DCUN1D1 (SCCRO) is required for spermatogenetic individualization. Plos one, 14(1), e0209995.

Xiao, J., Li, G., Zhou, J., Wang, S., Liu, D., Shu, G., ... & Ren, F. (2018). MicroRNA-520b functions as a tumor suppressor in colorectal cancer by inhibiting defective in cullin neddylation 1 domain containing 1 (DCUN1D1). Oncology research, 26(4), 593.

Zhong, J., Yuan, H., Xu, X., & Kong, S. (2018). MicroRNA‑195 inhibits cell proliferation, migration and invasion by targeting defective in cullin neddylation 1 domain containing 1 in cervical cancer Retraction in/10.3892/ijmm. 2021.4973. International Journal of Molecular Medicine, 42(2), 779-788.

Jiang, Y., Hou, R., Li, S., Li, S., & Dang, G. (2018). MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1. Experimental and therapeutic medicine, 16(2), 1000-1008.

Zhang, Z. H., Li, J., Luo, F., & Wang, Y. S. (2017). Clinical significance of SCCRO (DCUN1D1) in prostate cancer and its proliferation-inhibiting effect on Lncap cells. Eur Rev Med Pharmacol Sci, 21(19), 4283-4291.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant DCUN1D1 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-DCUN1D1 Recombinant Antibody (4B5) (CAT#: V2LY-0125-LY1261)

Mouse Anti-DCUN1D1 Recombinant Antibody (3D7) (CAT#: CBMAB-D0422-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry