Human Recombinant EphB4 protein, Biotin Conjugated, His Tag (V2LY-0526-LY3907)

Name: Human Recombinant EphB4 protein, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY3907
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant EphB4 protein, Biotin Conjugated, His Tag consist of Amino Acid: 1-539 and predicts a molecular mass of 58.5 kDa.

Molecule Mass

58.5 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-539

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

EPH Receptor B4

Research Area

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

Biological Process

Angiogenesis Source: UniProtKB
Axon guidance Source: GO_Central
Cell adhesion Source: UniProtKB
Cell migration involved in sprouting angiogenesis Source: UniProtKB
Ephrin receptor signaling pathway Source: UniProtKB
Heart morphogenesis Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Protein autophosphorylation Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Lymphatic malformation 7 (LMPHM7):
A form of primary lymphedema, a disease characterized by swelling of body parts due to developmental anomalies and functional defects of the lymphatic system. Patients with lymphedema may suffer from recurrent local infections. LMPHM7 is an autosomal dominant form with variable expressivity. Some individuals present with severe non-immune hydrops fetalis, which may cause perinatal demise or fully resolve after the neonatal period. Others present with no edema and have milder clinical features, such as atrial septal defect or varicose veins as adults.
Capillary malformation-arteriovenous malformation 2 (CMAVM2):
An autosomal dominant disorder characterized by multiple, round to oval or more irregularly shaped macules that are pinkish red in color and are randomly distributed across the body. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.

Topology

Extracellular: 16-539
Helical: 540-560
Cytoplasmic: 561-987

PTM

Phosphorylated; autophosphorylation is stimulated by EFNB2.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EphB4 protein, GST Tag (CAT#: V2LY-0526-LY3903)

Human Recombinant EphB4, Active protein, GST Tag (CAT#: V2LY-0526-LY3904)

Human Recombinant EphB4 protein, hFc Tag (CAT#: V2LY-0526-LY3905)

Human Recombinant EphB4 protein, His Tag (CAT#: V2LY-0526-LY3908)

Human Recombinant EphB4 (I782S) protein, GST Tag (CAT#: V2LY-0526-LY3909)

Human Recombinant EphB4 protein (CAT#: V2LY-0526-LY3910)

Mouse Recombinant EphB4 protein, hFc Tag (CAT#: V2LY-0526-LY8255)

Mouse Recombinant EphB4 protein, His Tag (CAT#: V2LY-0526-LY8256)

Human Recombinant EphB4 , Active protein, His Tag (CAT#: V2LY-0526-LY3906)

Human EPHB4 ELISA Kit (CAT#: V2LY-0626-LY4552)

Related Products

Human Recombinant EphB4 protein, His Tag (CAT#: V2LY-0526-LY3908)

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Mouse Anti-EPHB4 Recombinant Antibody (38) (CAT#: CBMAB-E1526-FY)

Human Recombinant EphB4 , Active protein, His Tag (CAT#: V2LY-0526-LY3906)

Human Recombinant EphB4 protein, hFc Tag (CAT#: V2LY-0526-LY3905)

Mouse Anti-EPHB4 Recombinant Antibody (CBFYE-0239) (CAT#: CBMAB-E0492-FY)

Mouse Anti-EphB4 Recombinant Antibody (CBT4356) (CAT#: V2LY-0625-LY2063)

Mouse Recombinant EphB4 protein, hFc Tag (CAT#: V2LY-0526-LY8255)

Rabbit Anti-EPHB4 Recombinant Antibody (D1) (CAT#: CBMAB-CP0619-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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