Human Recombinant GMFG protein, His Tag (V2LY-0526-LY4367)

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Basic Information

Expressed Host
E. coli
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant GMFG protein, His Tag consist of Amino Acid: 1-142 and predicts a molecular mass of 19 kDa.
Molecule Mass
19 kDa
Sequence
Amino Acid: 1-142
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>85% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
glia maturation factor gamma
Biological Process
Actin filament debranching Source: GO_Central
Negative regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
Protein phosphorylation Source: ProtInc
Regulation of actin cytoskeleton reorganization Source: InterPro
Cellular Location
Extracellular region; Ficolin-1-rich granule lumen; Secretory granule lumen

Tang, H., Liu, J., & Huang, J. (2022). GMFG (glia maturation factor gamma) inhibits lung cancer growth by activating p53 signaling pathway. Bioengineered, 13(4), 9284-9293.

Liu, J., Zhu, X., Gao, L., Geng, R., Tao, X., Xu, H., & Chen, Z. (2022). Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas. Frontiers in Molecular Neuroscience.

Li, Y., Tang, Y., Liu, J., Meng, X., Wang, Y., Min, Q., ... & Wang, J. Y. (2022). Glia maturation factor-γ is involved in S1P-induced marginal zone B-cell chemotaxis and optimal IgM production to type II T-independent antigen. International immunology, 34(1), 35-43.

Lan, A., Ren, C., Wang, X., Tong, G., & Yang, G. (2021). Bioinformatics and survival analysis of glia maturation factor-γ in pan-cancers. BMC cancer, 21(1), 1-10.

Li, Y., Tang, Y., Liu, J., Meng, X., Wang, Y., Min, Q., ... & Wang, J. Y. (2021). Glia maturation factor-γ is involved in S1P-induced marginal zone B cell chemotaxis and optimal T-independent type II antigen-induced IgM production. International Immunology.

Zia, S., Baqri, N., & Shahid, R. (2020). Glia maturation factor gamma, is a novel diagnostic marker of leukemia, has TAL1 binding sites in its promoter. Journal of King Saud University-Science, 32(1), 511-517.

Aerbajinai, W., Ghosh, M. C., Liu, J., Kumkhaek, C., Zhu, J., Chin, K., ... & Rodgers, G. P. (2019). Glia maturation factor-γ regulates murine macrophage iron metabolism and M2 polarization through mitochondrial ROS. Blood advances, 3(8), 1211-1225.

Aerbajinai, W., Zhu, J., Kumkhaek, C., Chin, K., & Rodgers, G. (2019). Glia Maturation Factor-γ Regulates Autophagy in Murine Macrophages. Blood, 134, 2318.

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For research use only. Not intended for any clinical use.

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