Human Recombinant GUCY2C protein, APC Conjugated, His Tag (V2LY-0526-LY4443)

Name: Human Recombinant GUCY2C protein, APC Conjugated, His Tag
SKU: V2LY-0526-LY4443
Rating: 5 (1 reviews)

See all GUCY2C antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GUCY2C protein, APC Conjugated, His Tag consist of Amino Acid: 1-430 and predicts a molecular mass of 49.8 kDa.

Molecule Mass

49.8 kDa

Conjugates

APC

Sequence

Amino Acid: 1-430

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Twelve months from date of receipt at -20°C to -70°C in lyophilized form and 3 months at -70°C under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

guanylate cyclase 2C (heat stable enterotoxin receptor)

Function

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP (PubMed:1718270, PubMed:11950846, PubMed:23269669, PubMed:22521417, PubMed:22436048).

Receptor for the E.coli heat-stable enterotoxin; E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GUCY2C (PubMed:1718270, PubMed:1680854).

Also activated by the endogenous peptides guanylin and uroguanylin (PubMed:8381596).

Biological Process

cGMP biosynthetic process Source: GO_Central
Intracellular signal transduction Source: InterPro
Receptor guanylyl cyclase signaling pathway Source: GO_Central
Regulation of cell population proliferation Source: Ensembl
Response to toxic substance Source: Ensembl
Signal transduction Source: GO_Central

Cellular Location

Cell membrane; Endoplasmic reticulum membrane. The 145 kDa plasma membrane form of GUCY2C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.

Involvement in disease

Diarrhea 6 (DIAR6):
A relatively mild, early-onset chronic diarrhea that may be associated with increased susceptibility to inflammatory bowel disease, small bowel obstruction, and esophagitis.
Meconium ileus (MECIL):
A condition characterized by intestinal obstruction due to inspissated meconium in the distal ileum and cecum, which develops in utero and presents shortly after birth as a failure to pass meconium. Meconium ileus is a known clinical manifestation of cystic fibrosis.

Topology

Extracellular: 24-430
Helical: 431-454
Cytoplasmic: 455-1073

PTM

Glycosylation at Asn-75 and/or Asn-79 is required for interaction with VIP36 while glycosylation at Asn-345 and Asn-402 modulates ligand-mediated GUCY2C activation.

Barton, J. R., Londregran, A. K., Alexander, T. D., Entezari, A. A., Bar-Ad, S., Cheng, L., ... & Waldman, S. A. (2023). Intestinal neuropod cell GUCY2C regulates visceral pain. The Journal of Clinical Investigation, 133(4).

Rappaport, J. A., Entezari, A. A., Caspi, A., Caksa, S., Jhaveri, A. V., Stanek, T. J., ... & Waldman, S. A. (2022). A β-catenin-TCF-sensitive locus control region mediates GUCY2C ligand loss in colorectal cancer. Cellular and Molecular Gastroenterology and Hepatology, 13(4), 1276-1296.

Lisby, A. N., Flickinger Jr, J. C., Bashir, B., Weindorfer, M., Shelukar, S., Crutcher, M., ... & Waldman, S. A. (2021). GUCY2C as a biomarker to target precision therapies for patients with colorectal cancer. Expert review of precision medicine and drug development, 6(2), 117-129.

Mishra, V., Bose, A., Kiran, S., Banerjee, S., Shah, I. A., Chaukimath, P., ... & Visweswariah, S. S. (2021). Gut-associated cGMP mediates colitis and dysbiosis in a mouse model of an activating mutation in GUCY2C. Journal of Experimental Medicine, 218(11).

van Vugt, A. H., Bijvelds, M. J., de Jonge, H. R., Meijsen, K. F., Restin, T., Bryant, M. B., ... & Middendorp, S. (2021). A potential treatment of congenital sodium diarrhea in patients with activating GUCY2C mutations. Clinical and Translational Gastroenterology, 12(11).

Jimenez-Luna, C., González-Flores, E., Ortiz, R., Martínez-González, L. J., Antúnez-Rodríguez, A., Expósito-Ruiz, M., ... & Prados, J. (2021). Circulating PTGS2, JAG1, GUCY2C and PGF mRNA in peripheral blood and serum as potential biomarkers for patients with metastatic colon cancer. Journal of Clinical Medicine, 10(11), 2248.

Mathur, D., Root, A. R., Bugaj-Gaweda, B., Bisulco, S., Tan, X., Fang, W., ... & Sapra, P. (2020). A Novel GUCY2C-CD3 T-Cell Engaging Bispecific Construct (PF-07062119) for the Treatment of Gastrointestinal CancersA GUCY2C-CD3 Bispecific Targets Gastrointestinal Cancers. Clinical Cancer Research, 26(9), 2188-2202.

Snook, A. E., Baybutt, T. R., Xiang, B., Abraham, T. S., Flickinger, J. C., Hyslop, T., ... & Waldman, S. A. (2019). Split tolerance permits safe Ad5-GUCY2C-PADRE vaccine-induced T-cell responses in colon cancer patients. Journal for ImmunoTherapy of Cancer, 7(1), 1-12.

Merlino, D. J., Barton, J. R., Charsar, B. A., Byrne, M. D., Rappaport, J. A., Smeyne, R. J., ... & Waldman, S. A. (2019). Two distinct GUCY2C circuits with PMV (hypothalamic) and SN/VTA (midbrain) origin. Brain Structure and Function, 224, 2983-2999.

Magee, M. S., Abraham, T. S., Baybutt, T. R., Flickinger Jr, J. C., Ridge, N. A., Marszalowicz, G. P., ... & Snook, A. E. (2018). Human GUCY2C-targeted chimeric antigen receptor (CAR)-expressing T cells eliminate colorectal cancer metastases. Cancer immunology research, 6(5), 509-516.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant GUCY2C protein, APC Conjugated, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY1255)

Cynomolgus Recombinant GUCY2C protein, His Tag (CAT#: V2LY-0526-LY1526)

Human Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY4440)

Human Recombinant GUCY2C protein, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY4441)

Human Recombinant GUCY2C protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY4442)

Human Recombinant GUCY2C protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY4444)

Mouse Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY8380)

Rat Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY9405)

Related Products

Human Recombinant GUCY2C protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY4444)

Cynomolgus Recombinant GUCY2C protein, His Tag (CAT#: V2LY-0526-LY1526)

Mouse Anti-GUCY2C Recombinant Antibody (CBXS-0983) (CAT#: V2LY-0226-LY2092)

Rat Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY9405)

Human Anti-GUCY2C Recombinant Antibody (3D5) (CAT#: CBMAB-G5791-LY)

Mouse Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY8380)

Human Anti-GUCY2C Recombinant Antibody (
2E5) (CAT#: V2LY-0725-LY739)

Canine Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY1255)

Human Recombinant GUCY2C protein, ECD, His Tag (CAT#: V2LY-0526-LY4440)

Human Recombinant GUCY2C protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY4442)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry