Human Recombinant HNMT protein, GST Tag (V2LY-0526-LY4565)

Name: Human Recombinant HNMT protein, GST Tag
SKU: V2LY-0526-LY4565
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant HNMT protein, GST Tag consist of Amino Acid: 1-292 and predicts a molecular mass of 60.5 kDa.

Molecule Mass

60.5 kDa

Sequence

Amino Acid: 1-292

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

histamine N-methyltransferase

Function

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.

Biological Process

Histamine catabolic process Source: UniProtKB
Histamine metabolic process Source: ARUK-UCL
Histidine catabolic process Source: Reactome
Methylation Source: UniProtKB
Respiratory gaseous exchange by respiratory system Source: ProtInc

Cellular Location

Cytoplasm

Involvement in disease

Mental retardation, autosomal recessive 51 (MRT51):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.

Otsuka, R., Naganuma, F., Nakamura, T., Miwa, H., Nakayama-Naono, R., Matsuzawa, T., ... & Yoshikawa, T. (2022). Contribution of astrocytic histamine N-methyltransferase to histamine clearance and brain function in mice. Neuropharmacology, 212, 109065.

Lu, Y., Dong, C. Z., Bao, D., Zhong, C., Liu, K., Chen, L., ... & Yang, B. (2022). The Thr105Ile Variant (rs11558538) of the Histamine N-methyltransferase Gene may be associated with Reduced Risk of Parkinson Disease: A Meta-analysis. Genetic Testing and Molecular Biomarkers, 26(11), 543-549.

Wang, G., Ogawa, F., O'Beirne, S. L., Salit, J., Strulovici-Barel, Y., Kaner, R. J., & Crystal, R. G. (2018). Smoking-Induced Constitutive Suppression of Histamine N-Methyltransferase Is Accompanied with Infiltration of Mast Cells in Human Small Airway Epithelium. In B93. WHERE THERE IS SMOKE, THERE IS LUNG DISEASE (pp. A4163-A4163). American Thoracic Society.

Jia, H. Z., Liu, S. L., Zou, Y. F., Chen, X. F., Yu, L., Wan, J., ... & Zhang, W. (2018). MicroRNA-223 is involved in the pathogenesis of atopic dermatitis by affecting histamine-N-methyltransferase. Cellular and Molecular Biology, 64(3), 103-107.

Chen, M. M., Xu, L. H., Chang, L., Yin, P., & Jiang, Z. L. (2018). Reduction of motion sickness through targeting histamine N-methyltransferase in the dorsal vagal complex of the brain. Journal of Pharmacology and Experimental Therapeutics, 364(3), 367-376.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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